Reciprocal Template Effects in Bisubstrate Systems: A Replication Cycle

Article abstract of DOI:10.1016/0040-4020(94)00910-M

We report the use of high affinity synthetic receptors for adenine derivatives as a basis for designed templated reactions.The rate of the coupling of two adenine derivatives, an active ester and an amine, is enhanced in the presence of a template bearing two receptor sites.In complementary synthetic reactions, the formation of the template, itself the coupling product from an active ester and an amine, was shown to be enhanced by the product of the first reaction.Thus reciprocal template effects are observed.Rate enhancements due to the formation of termolecular complexes were up to 13 fold.Experimentally observed rate enhancements correlated well with calculated concentrations of the termolecular complex.In addition, large rate accelerations (1E3) were observed due to bimolecular complexes in the coupling of two components, one of which contains a receptor for the other.