Synthetic nucleoside and nucleotides. XXXIV. Photoaffinity labeling of HIV reverse transcriptase: Synthesis and utilization of 2',3'-dideoxy uridylate analogs bearing aryl)trifluoromethyl)-diazirine moiety

Article abstract of DOI:10.1080/07328319608002409

In order to develop a photoaffinity labeling reagent for HIV-1 reverse transcriptase, a 2',3'-dideoxyUTP analog bearing a photo-reactive group at the 5-position of the uracil ring, 2',3'-dideoxy-E-5-[4-[3-(trifluoromethyl)-3H-diazirin-3-yl]styryl]UTP, was designed and synthesized. This photo-reactive analog could preferentially bind to the 66 kDa subunit of the p66/p51 heterodimeric HIV-1 reverse transcriptase under irradiation by near-ultraviolet light (365 nm).

Full text of DOI:10.1080/07328319608002409

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Synthetic Nucleoside and Nucleotides.
XXXIV. Photoaffinity Labeling of HIV
Reverse Transcriptase: Synthesis and
Utilization of 2′,3′-Dideoxy Uridylate
Analogs Bearing Aryl(trifluor0methyl)-
Diazirine Moiety
Toyofumi Yamaguchi a & Mineo Saneyoshi a
a Department of Biological Sciences , The Nishi-Tokyo
University , 2525 Yatsuzawa, Uenohara-machi, Kitatsuru-gun,
Yamanashi, 409-01, Japan
Published online: 21 Aug 2006.
To cite this article: Toyofumi Yamaguchi & Mineo Saneyoshi (1996) Synthetic Nucleoside and
Nucleotides. XXXIV. Photoaffinity Labeling of HIV Reverse Transcriptase: Synthesis and Utilization
of 2′,3′-Dideoxy Uridylate Analogs Bearing Aryl(trifluor0methyl)-Diazirine Moiety , Nucleosides
and Nucleotides, 15:1-3, 607-618
To link to this article: http://dx.doi.org/10.1080/07328319608002409
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