Fluorescent molecular probes I. The synthesis and biological properties of an ELF β-glucuronidase substrate that yields fluorescent precipitates at the enzymatic activity sites

Article abstract of DOI:10.1016/S0040-4020(97)00413-4

A novel quinazolinone-based fluorogenic β-glucuronidase substrate has been synthesized and evaluated. In this protocol, a partially protected salicyl glucuronide is oxidatively condensed with anthranilamide to afford the required quinazolinonyl glucuronic acid methyl ester that is readily deprotected to yield the desired glucuronide, known as ELF-97 glucuronide. The glucuronide is a sensitive fluorogenic β-glucuronidase substrate that yields a bright yellow-green fluorescent precipitate upon enzymatic reaction.