Nucleosides and Nucleotides p. 1369 - 1378 (1997)
Update date:2022-08-05
Topics:
Charubala, Ramamurthy
Maurinsh, Juris
Roesier, Angelika
Melguizo, Manuel
Jungmann, Oliver
Gottlieb, Margarete
Lehbauer, Joerg
Hawkins, Mary
Pfleiderer, Wolfgang
Chemical syntheses of 1-(2-deoxy-β-D-ribofuranosyl)lumazines and isoptefins as well as 8-(2-deoxy-β-D-ribofuranosyl)-4-amino-7(8H)pteridones and -isoxantho-pterins have been developed to make the structural analogs of the naturally occurring 2'-deoxyribonucleosides in the pteridine series available. The corresponding phosphoramidites have been used in machine- aided solid-support syntheses leading to new types of fluorescence labeled oligonucleotides. The effects of the various fluorophors on duplex formation and as labels for enzyme reactions is demonstrated.
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