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22. Enzyme assay was performed at 25 °C on a PerkinElmer VICTOR plate reader
with the excitation and emission wavelengths at 405 and 530 nm, respectively.
The assay were run in 200
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EDTA, 10% sucrose and 10 Ac-DEVD-AFC. The final concentration of
l
L volumes and contained the following: 25 mM K+
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v
versus [S] data imnto
v = Vmax[S]/(Km + S), using BioDataFit (http://
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