A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

Article abstract of DOI:10.1016/S0040-4039(99)00972-7

Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.