Microscopic protonation constants in tobramycin. An NMR and pH study with the aid of partially N-acetylated derivatives

Article abstract of DOI:10.1016/0008-6215(93)84244-Z

Three tetra-N-acetyl derivatives and one tri-N-acetyl derivative of tobramycin (1) have been prepared by partial N-acetylation.Comparison of the pK_a values, determined by NMR chemical shift titrations and pH titration of the derivatives, with t