Journal of labelled compounds and radiopharmaceuticals p. 295 - 304 (2001)
Update date:2022-08-03
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The synthesis of two selectively labeled isotopomers of L-phenylalanine, (Phe), using chemical and enzymatic methods is reported. The [3R-3H]-L-Phe isotopomer has been obtained from [3-3H]cinnamic acid prepared from benzaldehyde and malonic acid using tritiated water as a source of radioactive label, and by addition of ammonia in the presence of enzyme PAL. The [3S-3H]-L-Phe isotopomer has been synthesized by addition of ammonia to cinnamic acid in a buffered medium containing PAL and tritiated water.
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