Optimizing the synthetic route of chromone-2-carboxylic acids: A step forward to speed-up the discovery of chromone-based multitarget-directed ligands

Article abstract of DOI:10.3390/molecules24234214

6-Bromochromone-2-carboxylic acid (3) was synthesized by a microwave-assisted process. The optimization of the reaction was performed varying parameters, such as type of base/number of reagent equivalents, solvent, temperature and reaction time. The yield of the reaction was improved to 87%. The new synthetic route is versatile as several chromone-2-carboxylic acids (compounds 4B-10B) were obtained with good yields (54-93%). Only in the case of the nitro substituent (compound 11B), an ester was obtained instead of the desired carboxylic acid. Following this synthetic route chromone carboxylic acids can be attained with a high degree of purity, without the need of the tedious and expensive purification processes through column chromatography. The reaction is safe, cost-effective, fast and robust, and can be used in the development of concise and diversity-oriented libraries based on chromone scaffold. The overall study can be looked as a step forward to speed-up the discovery of chromone-based multitarget-directed ligands.

Full text of DOI:10.3390/molecules24234214

molecules
Article
Optimizing the Synthetic Route of
Chromone-2-carboxylic Acids: A Step forward to
Speed-up the Discovery of Chromone-based
Multitarget-directed Ligands
Fernando Cagide *, Catarina Oliveira, Joana Reis † and Fernanda Borges *
CIQUP/Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo
Alegre, 4169-007 Porto, Portugal; catarina.oliveira.fc@gmail.com (C.O.); jocostareis@gmail.com (J.R.)
* Correspondence: fernando.fagin@fc.up.pt (F.C.); fborges@fc.up.pt (F.B.)
† Present address: Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1,
27100 Pavia, Italy.
Academic Editors: Ionel Mangalagiu and John Spencer
Received: 13 September 2019; Accepted: 19 November 2019; Published: 20 November 2019
ꢀꢁꢂꢀꢃꢄꢅꢆꢇ
ꢀꢁꢂꢃꢄꢅꢆ
Abstract: 6-Bromochromone-2-carboxylic acid (3) was synthesized by a microwave-assisted process.
The optimization of the reaction was performed varying parameters, such as type of base/number of
reagent equivalents, solvent, temperature and reaction time. The yield of the reaction was improved to
87%. The new synthetic route is versatile as several chromone-2-carboxylic acids (compounds 4B–10B)
were obtained with good yields (54–93%). Only in the case of the nitro substituent (compound 11B),
an ester was obtained instead of the desired carboxylic acid. Following this synthetic route chromone
carboxylic acids can be attained with a high degree of purity, without the need of the tedious and
expensive purification processes through column chromatography. The reaction is safe, cost-effective,
fast and robust, and can be used in the development of concise and diversity-oriented libraries based
on chromone scaffold. The overall study can be looked as a step forward to speed-up the discovery of
chromone-based multitarget-directed ligands.
Keywords: chromone-2-carboxylic acids; microwave-assisted reaction; multitarget ligands
1. Introduction
Chromones are oxygen-containing heterocyclic compounds with a benzoannelated
γ-pyrone
ring widely distributed in nature and present in notable amounts in several species [ ]. They are
1,2
well-known by their diversity of pharmacological properties, such as antiallergic, anti-inflammatory,
antidiabetic, antitumor, and antimicrobial [2].
Chromone (4H-chromen-4-one; 4H-1-benzopyran-4-one) is a privileged structure used in drug
discovery programs, being the scaffold recognized as a pharmacophore of a great number of bioactive
molecules [
characterized as multifactorial pathologies, the development of multitarget-directed ligands is a
hot drug discovery issue [ ]. In this context, following a multitarget strategy, our research group
1–3]. As neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease, are
4
have developed chromone-based multitarget ligands as bifunctional acetylcholinesterase (AChE) and
monoamine oxidase (MAO) inhibitors (Figure 1) [5].
Molecules 2019, 24, 4214; doi:10.3390/molecules24234214
www.mdpi.com/journal/molecules

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Figure 1. Drug design strategy followed to obtain multitarget ligands based on chromone scaffold.
Within this framework the process development and chemical route scouting for the library
generation is of utmost significance. Synthetic routes must be optimized in terms of time, amount
of by-products, environmental impact, yield, number of purification steps, among other parameters.
In addition, the use of green and sustainable chemistry is also an important issue.
Microwave technology has been used in chemistry since the late 1970s, but it has only been
implemented in organic synthesis since the mid-1980s. However, in the past few years, heating chemical
reactions by microwave energy has been progressively a more popular subject [6].
The availability of safe, single-mode dedicated MW apparatus has allowed the incorporation
of this technology into accelerating hit-to-lead, and lead optimization programs [7–9]. Their use
dramatically reduces processing times, increase product yields, and enhance the purity of the product,
when compared to the conventional experiments [3].
The functionalization of the chromone scaffold originates remarkable derivatives, namely those
possessing a reactive carbonyl group (e.g., carboxylic acid), which are versatile synthons owing to its
ability to participate in an extensive spectrum of chemical reactions [1,3,10].
In this work, our main goal was to develop an effective and fast synthetic route to obtain
4-oxo-4H-chromene-2-carboxylic acid and its derivatives, which are often used in medicinal chemistry
to develop small libraries for structure-activity studies (Figure 2). Till now few MW reactions for the
novo synthesis of this type of building blocks has been described [11–13].
Figure 2. Chromones obtained from 4-oxo-4H-chromene-2-carboxylic acid with relevant biological
activity [1,2].
In this context, we started the MW studies with the commercial compound
50-bromo-20-hydroxyacetophenone to obtain 6-bromochromone-2-carboxylic acid (
3, Scheme 1).
Although this chromone is commercially available it is very costly for drug discovery

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programs. After reaction optimization, the study was extended to the synthesis of other
4-oxo-4H-chromene-2-carboxylic acid derivatives in order to obtain the suitable synthons to speed-up
the progress on the discovery of chromone-based multitarget-directed ligands.
Scheme 1. General synthetic strategy followed to obtain compound 3.
2. Results and Discussion
The synthesis of 6-bromochromone-2-carboxylic acid (3) was initially chosen for the optimization
study. As the chromone is commercially available it was used as standard allowing a rapid confirmation
of its formation along the reaction by thin-layer chromatography (TLC).
The synthesis of 6-bromochromone-2-carboxylic acid
(
3
)
was carried out using
50-bromo-20-hydroxyacetophenone (
1) as starting material and reaction conditions similar to the
classic reaction described in the literature [14].
Herein we describe a method to obtain 6-bromochromone-2-carboxylic acid (3) by a MW-assisted
synthesis (Scheme 1) comprising two steps: (1) addition of ethyl oxalate (compound
2) and a base; (2)
addition of chloridric acid (HCl) and the studies performed for its optimization, which include diverse
parameters-type of base/number of reagent equivalents, solvent, temperature and reaction time.
The study was started with the conditions described in Table 1, Entry 1: sodium ethoxide
(EtONa, base, 1 equiv.) in ethanol at 120 ◦C and 3 mL of HCl, 1 M for the hydrolysis step. The associated
times of the steps (1) and (2) (Time 1 and 2, respectively) were defined as 10 min. With these conditions
a low yield (14%) was obtained (Table 1, Entry 1). When the base was replaced by sodium methoxide
(NaOMe) in methanol (Table 1, Entry 2) similar results were obtained. To note that these strong
bases are commercially available in alcoholic solutions, and used as such. Based on the data, and our
experience on the synthesis of chromones, NaOMe was the base chosen for the following studies as it
is slightly cheaper, more stable and fewer side products were observed by TLC.
The increase of the equivalents number of the base (NaOMe) and of the ethyl oxalate lead to an
improvement of the yield of compound
3 (Table 1, Entries 3–6), being the best reactional conditions
attained with two equivalents of NaOMe and three of ethyl oxalate, with a yield of 21% (Table 1, Entry 6).
After that, the effect of the acid concentration used in the step 2) was studied (Table 1, Entries 7–8).
Although a change of 1 M to 6 M was performed, the yield up only to 30% (Table 1, Entry 8).
Additional modifications in the temperature and time of the reaction were performed. The increase
of the temperature of the first reaction step to 140 ◦C resulted in a decrease of the yield to 19% (Table 1,
Entry 9). However, keeping the temperature at 120 ◦C and increasing the reaction time of the step
(1) to 20 min, a small increment of the yield to 34% was observed (Table 1, Entry 10). Nevertheless,
longer times of reaction (30 min) did not produce any performance upsurge (Table 1, Entry 11).
To improve the acetophenone solubilization the solvent of the reaction was also changed to a less
polar (Table 1, Entries 12,13). The most significant results were obtained when dioxane was used with
an increase in yield going from 34% to 68% (Table 1, Entry 13).

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Table 1. Microwave-assisted optimization conditions for the synthesis of compound 3.
Base
(Equiv)
2
T
Time 1 *
(min)
Acid
(V = 3 mL)
Time 2 *
(min)
Yield
(%)
Entry
Solvent
(Equiv)
(◦C)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
EtONa (1)
MeONa (1)
MeONa (2)
MeONa (3)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
MeONa (2)
1
1
1
1
2
3
3
3
3
3
3
3
3
3
3
3
EtOH
MeOH
MeOH
MeOH
MeOH
MeOH
MeOH
MeOH
MeOH
MeOH
MeOH
iPrOH
120
120
120
120
120
120
120
120
140
120
120
120
120
120
120
120
10
10
10
10
10
10
10
10
10
20
30
20
20
20
20
20
HCl (1 M)
HCl (1 M)
HCl (1 M)
HCl (1 M)
HCl (1 M)
HCl (1 M)
HCl (3 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (6 M)
HCl (1 M)
10
10
10
10
10
10
10
10
10
10
10
10
10
30
40
50
14
15
19
17
19
21
23
30
19
34
34
51
68
81
87
85
Dioxane
Dioxane
Dioxane
Dioxane
* The temperature was maintained for the two reaction steps (1) and (2) (Time 1 and 2, respectively).
The final optimization step was related with the increment of the hydrolysis time of the ester
(step 2, Table 1, Entries 14,15): 40 min allowed to obtain compound 3 with a yield of 87% (Table 1,
Entry 15). Likewise, longer times of reaction (50 min) did not produce an additional increase of the
performance of the reaction (Table 1, Entry 16).
After the reaction optimization its versatility was verified using dissimilar 20-hydroxyacetophenone
derivatives (A) to obtain chromone-2-carboxylic acid derivatives (B) having different type of substituents
(Table 2). The selected substituents are often used in medicinal chemistry programs [15,16].
When the 20-hydroxyacetophenone without substituents in the aromatic ring (compound 4A
)
was used, a decrease in yield (compound 4B, 54%, Table 2) was observed in comparison with the
obtained 6-bromochromone-2-carboxylic acid (compound , 87%, Table 1, Entry 15). The replacement
3
of bromine by a chlorine in the aromatic ring (compound 5A) allowed to obtain the desired chromone
with a slight lower yield (compound 5B, 71%, Table 2). A similar behavior was observed when the
20-hydroxyacetophenone substituent was a methyl group (compound 6B, 64%, Table 2). When the
acetophenone is substituted with one methoxyl group (compounds 7A
,
8A and 9A) a similar reaction
8B and 9B, Table 2) was
performance to compound , with yields between 81–93% (compounds 7B
3
,
obtained. However, when the 20-hydroxyacetophenone has two methoxy substituents in positions 7
and 8 (compound 10A) a reduction of the reaction yield to 62% was observed (compound 10B, Table 2).
As all the purification steps were based only on the washing process, without further purification
by column chromatography, a loss of mass along the process was, in general, observed.
Finally, when the aromatic ring possesses a nitro group in position 6 (compound 11A), instead of
the desired chromone carboxylic acid, the corresponding methyl ester was obtained. This shortcoming
can be related to the presence of a highly deactivating substituent that can preclude ester hydrolysis
by stabilizing the reaction intermediate or by bring about a compound with low solubility in the
reaction media.

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Table 2. Synthesis of chromone-2-carboxylic acids by microwave-assisted reaction.
20-Hydroxyacetophenone Chromone-2-Carboxylic
(A) Acid (B)
Compound
4
Yield (%)
54 a
5
6
71
64
7
8
83
81
9
93
10
11
62
83
a. The yield of the compound has been previously described, for similar reaction conditions and with a domestic
MW apparatus, as 93% [17].

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3. Material and Methods
3.1. Apparatus
Microwave-assisted synthesis was performed in a Biotage® Initiator Microwave Synthesizer
(Biotage, Uppsala, Sweden). 1H- and 13C-NMR spectra were recorded on an AMX 400 NMR
spectrometer (Bruker, Billerica, MA, USA). Spectra were recorded at room temperature in 5 mm outside
diameter (o.d.) tubes. Tetramethylsilane (TMS) was used as internal standard, chemical shifts are
expressed in ppm (
δ
) and J in Hz. For the DEPT sequence, the width of the 90◦ pulse for 13C was
s; the delay 2JC,H 1 was set to 3.5 ms (underlined
4
µ
s, and that of the 90◦ pulse for 1H was 9.5
µ
−
values). The melting point were carried out on a Stuart Scientific Melting Point SMP1 apparatus
(Stuart Scientific, Staffordshire, UK).
3.2. Reagents and General Conditions
All reagents were purchased from Sigma-Aldrich Química S.A. (Lisbon, Portugal) and
TCI Chemicals Europe (Paris, French). All solvents were pro analysis grade from Merck
(Darmstadt, Germany), Carlo Erba Reagents (Val de Reuil, France) and Scharlab (Barcelona, Spain).
Thin layer chromatography (TLC) was performed on precoated silica gel 60 F254 acquired from
Merck with layer thickness of 0.2 mm. Reaction control was monitored using ethyl acetate and/or ethyl
acetate:methanol (9:1) and spots were visualized under UV detection at 254 and 366 nm.
3.3. Synthesis of 4-Oxo-4H-chromene-2-carboxylic acids 3–11
General Procedure
The appropriate hydroxyacetophenone (
MW vial. Then diethyl oxalate (3.49 mmol, 474
531
1
or
µ
A
, 1.16 mmol) was dissolved in dioxane (2 mL) in a
L) and a solution of MeONa in MeOH (2.32 mmol,
µ
L, 25% w/w) were added. The resulting solution was heated to 120 ◦C for 20 min. Then, a solution
of HCl (18 mmol, 3 mL, 6 M) was added and the reaction was heated to 120 ◦C for 40 min. The reaction
was decanted over water (50 mL) and the solid formed was filtered and washed with water. The solid
was then dried, washed with dichloromethane and dried again.
1
6-Bromo-4-oxo-4H-chromene-2-carboxylic acid (
3):
η
= 87%, H-NMR (DMSO-d6)
δ
: 8.12 (1H, d,
J = 2.4 Hz, H5), 8.03 (1H, dd, J1 = 9.0 Hz, J2 = 2.5 Hz, H7), 7.74 (1H, d, J = 9.0 Hz,), 6.95 (1H, s, H3).
13C-NMR (DMSO-d6)
176.4 (C4), 160.9 (COOH), 154.4 (C2), 153.6 (C8a), 137.7 (C6), 126.9 (C5), 125.2
(C4a), 121.5 (C3), 118.4 (C6), 113.4 (C8). M.p.: 263–267 ◦C.
4-Oxo-4H-chromene-2-carboxylic acid (4B):
= 54%, 1H-NMR (MeOD)
δ
η
δ: 8.17 (1H, dd, J1 = 8.0 Hz,
J2 = 1.7 Hz, H5), 7.88 (1H, ddd, J1 = 8.7 Hz, J2 = 7.1 Hz, J3 = 1.7 Hz, H7), 7.72 (1H, dd, J1 = 8.7, J1
= 1.1 Hz, H8), 7.55 (1H, ddd, J1 = 8.0 Hz, J2 = 7.1 Hz, J3 = 1.1 Hz, H6), 7.08 (1H, s, H3). 13C-NMR
(MeOD-d4) δ:179.4 (C4), 161.5 (COOH), 156.2 (C2), 153.9 (C8a), 135.1 (C7), 125.9 (C5), 124.9 (C6), 123.8
(C4a), 118.6 (C3), 113.3 (C8). M.p.: >285 ◦C dec.
6-Chloro-4-oxo-4H-chromene-2-carboxylic acid (5B):
η
= 71%, 1H-NMR (DMSO-d6)
δ: 8.08 (1H, d,
J = 2.6 Hz, H5), 7.83 (1H, dd, J1 = 9.0, J2 = 2.6 Hz, H7), 7.71 (1H, d, J = 9.0 Hz, H8), 7.06 (1H, s, H3).
13C-NMR (DMSO-d6)
: 176.6 (C4), 161.2 (COOH), 154.1 (C2), 153.6 (C8a), 135.0 (C7), 130.5 (C6), 124.8
(C5), 123.9 (C2a), 121.4 (C3), 113.3 (C8). M.p.: 280–284 ◦C.
6-Methyl-4-oxo-4H-chromene-2-carboxylic acid (6B):
= 64%, 1H-NMR (400 MHz, MeOD)
(1H, d, J = 2.3 Hz, H5), 7.68 (1H, dd, J1 = 8.7, J2 = 2.3 Hz, H6), 7.58 (1H, d, J = 8.6 Hz, H8), 7.02 (1H, s,
H3), 2.48 (3H, s, CH3). 13C-NMR (MeOD-d4)
: 180.8 (C4), 163.0 (COOH), 156.0 (C2), 155.1 (C8a), 137.8
(C7), 137.7 (C6), 125.5 (C5), 125.0 (C4a), 119.8 (C3), 114.6 (C8), 21.0 (CH3). M.p.: >279 ◦C dec.
7-Methoxy-4-oxo-4H-chromene-2-carboxylic acid (7B): : 7.95 (1H, d,
= 83%, 1H-NMR (DMSO-d6)
J = 9.2 Hz, H5), 7.22 (1H, d, J = 2.4 Hz, H8), 7.12 (1H, dd, J1 = 9.2 Hz, J2 = 2.4 Hz, H6), 6.86 (1H, s, H3),
3.94 (3H, s, OCH3). 13C-NMR (DMSO-d6)
: 177.2 (C4), 165.1 (C7), 161.9 (COOH), 158.0 (C2), 153.6
(C8a), 126.9 (C5), 118.2 (C4a), 116.1 (C3), 114.2 (C6), 101.5 (C8), 56.8 (OCH3). M.p.: 283–287 ◦C.
δ
η
δ: 7.92
δ
η
δ
δ

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6-Methoxy-4-oxo-4H-chromene-2-carboxylic acid (8B):
η
= 81%, 1H-NMR (DMSO-d6)
δ: 7.71 (1H, d, J
= 9.2 Hz, H8), 7.47 (1H, dd, J1 = 9.2 Hz, J1 = 3.2 Hz, H7), 7.42 (1H, d, J = 3.2 Hz, H5), 6.89 (1H, s, H3),
3.88 (3H, s, OCH3). 13C-NMR (DMSO-d6)
: 177.3 (C4), 161.5 (COOH), 157.0 (C2), 153.0 (C6), 150.2
(C8a), 124.5 (C4a), 124.4 (C7), 120.5 (C3), 112.5 (C8), 104.5 (C5), 55.8 (OCH3). M.p.: 293–294 ◦C.
5-Methoxy-4-oxo-4H-chromene-2-carboxylic acid (9B): : 7.73 (1H, dd,
= 93%, 1H-NMR (MeOD)
J1 = 8.5 Hz, J2 = 8.5 Hz, 7H), 7.20 (1H, dd, J = 8.5, J2 = 0.9 Hz, H8), 7.03 (1H, dd, J = 8.5, J2 = 0.9 Hz,
H6), 6.91 (1H, s, H3), 3.95 (3H, s, OCH3). 13C-NMR (MeOD-d4)
: 179.1 (C4), 161.4 (COOH), 159.6
δ
η
δ
δ
(C2), 158.1 (C5), 151.8 (C8a), 135.4 (C7) 115.0 (C3), 114.2 (C4a), 110.1 (C6), 107.0 (C8), 55.3 (OCH3).
M.p.:> 256◦C dec.
7,8-Dimethoxy-4-oxo-4H-chromene-2-carboxylic acid (10B):
d, J = 9.0 Hz, H5), 7.31 (1H, d, J = 9.0 Hz, H6), 6.82 (1H, s, H3), 3.95 (3H, s, OCH3), 3.91 (3H, s, OCH3).
13C-NMR (DMSO-d6)
: 176.9 (C4), 161.4 (COOH), 156.8 (C2), 152.9 (C7), 149.7 (C8a), 136.5 (C8), 120.1
(C3), 118.3 (C4a), 112.9 (C5), 111.4 (C7), 60.9 (OCH3), 56.5 (OCH3). M.p.: >287 ◦C dec.
Methyl 6-nitro-4-oxo-4H-chromene-2-carboxylate (11B):
= 83%, 1H-NMR (DMSO-d6)
J = 2.8 Hz, H6), 8.61 (1H, dd, J1 = 9.2, J2 = 2.8 Hz, H7), 8.00 (1H, d, J = 9.2 Hz, H7), 7.03 (1H, s, H3), 3.57
η δ: 7.77 (1H,
= 62%, 1H-NMR (DMSO-d6)
δ
η
δ: 8.72 (1H, d,
(3H, s, OCH3). 13C-NMR (DMSO-d6)
δ: 176.9 (C4), 160.9 (COOCH3), 158.5 (C2), 154.0 (C8a), 144.7 (C6),
129.2 (C7), 123.7 (C4a), 121.2 (C5), 120.9 (C3), 113.7 (C8), 66.3 (OCH3). M.p.: 274–279 ◦C.
Details of apparatus and NMR spectra of the synthesized compounds are available in
Supplementary Materials.
4. Conclusions
The synthesis of 6-bromochromone-2-carboxylic acid (
) as starting material and ethyl oxalate ( ) was successfully accomplished using a MW-assisted process.
3
) using 50-bromo-20-hydroxy-acetophenone
(1
2
For the optimization process a number of parameters were adjusted, such as type of base/number of reagent
equivalents, solvent, temperature and reaction time. The yield of the reaction was improved to 87%.
The new synthetic route is versatile as several chromone-2-carboxylic acids (compounds 4B–10B
were obtained with good yields (54–93%). Only in the case of the nitro substituent (compound 11B),
an ester was obtained instead of the desired carboxylic acid.
)
Following this synthetic route, a variety of chromone carboxylic acids can be attained with a high
degree of purity, without the need of the tedious and expensive purification processes through column
chromatography. This is a great advantage for the scale-up of the process.
The reaction is safe, cost-effective, fast, robust, and can be used in the development of concise and
diversity-oriented libraries based on chromone scaffold. The overall study can be looked as a step
forward to speed-up the progress of chromone-based multitarget-directed ligands.
Supplementary Materials: The following are available online, Details apparatus and NMR spectra of the
synthesized compounds can be found in the Supporting Information.
Author Contributions: F.C., C.O., J.R., F.B.: Designed the project, synthesized the compounds, analysed the data
and wrote the paper. All authors revised the manuscript and agreed with submission.
Funding: This work was funded by FEDER funds through the Operational Programme Competitiveness
Factors-COMPETE and national funds by FCT–Foundation for Science and Technology under research grants
UID/QUI/00081/2019 and FEDER/COMPETE POCI-01-0145-FEDER-028397. This article is based upon work from
COST Action CA15135.
Conflicts of Interest: The authors declare no conflict of interest.
Abbreviations
AChE
MAO
MW
Acetylcholinesterase
Monoamine oxidase
Microwave
TLC
Thin-layer chromatography

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Sample Availability: Samples of the compounds are available from the authors.
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