V. Kiss et al. / Tetrahedron: Asymmetry 17 (2006) 2220–2234
2233
afford (ꢀ)-1-(3-chloro-phenyl)-2-propanol maleic acid
monoester (ꢀ)-11 (10.22 g, 38.04 mmol, Y: 63.9%).
filtered, washed with hexane (3 · 2 mL) and dried to afford:
(ꢀ)-1-(3,4-dichloro-phenyl)-2-propanol
(ꢀ)-5
(4.43 g,
25
21.60 mmol, Y: 65.6%), white crystals, {½aꢁD ¼ ꢀ23:1 (c
The monoester was removed: (ꢀ)-1-(3-chloro-phenyl)-2-
propanol maleic acid monoester (ꢀ)-11 (10.22 g) was
heated with a mixture of water (20 mL) and NaOH (5 g)
for 1 min and it was allowed to cool to room temperature.
The solution was extracted with diethyl ether (4 · 20 mL).
The combined organic phase was dried over Na2SO4 and
the solvent was removed in vacuo to afford (ꢀ)-1-(3-
2, CHCl3)}, ee: 80%.
The mother liquid was evaporated: (ꢀ)-1-(3,4-dichloro-
phenyl)-2-propanol (ꢀ)-5 (2.27 g, 11.07 mmol, Y: 33.6%),
25
{½aꢁD ¼ ꢀ7:8 (c 2, CHCl3)}, ee: 27%.
chloro-phenyl)-2-propanol (ꢀ)-3 (6.08 g, 35.63 mmol, Y:
Acknowledgements
25
59.8%) {½aꢁD ¼ ꢀ11:7 (c 2, CHCl3)}, ee: 34%.
The Hungarian OTKA Foundation (Project No. T042725
E.F.) is gratefully acknowledge for financial support.
V.K. thanks Gedeon Richter Ltd, for doctoral fellowship.
4.15. Purification by recrystallization of the enantiomers
4.15.1. Purification of (ꢀ)-1-benzo[1,3]dioxol-5-yl-2-propa-
nol maleic acid monoester (ꢀ)-8 enantiomer with recrystal-
lization. (ꢀ)-1-Benzo[1,3]dioxol-5-yl-2-propanol maleic
acid monoester (ꢀ)-8 (10.00 g, 35.94 mmol, ee: 48%) was
dissolved in toluene (20.0 mL) with heating. The solution
was inoculated with pure (ꢀ)-1-benzo[1,3]dioxol-5-yl-2-
propanol maleic acid monoester (ꢀ)-8 and stirred for 3 h
at room temperature. The crystals were filtered, washed
with toluene (3 · 2 mL) and hexane (3 · 2 mL), and dried
to afford: (ꢀ)-1-benzo[1,3]dioxol-5-yl-2-propanol maleic
References
1. Newmann, P. In Optical Resolution Procedures for Chemical
Compounds; Optical Resolution Information Center, Man-
hattan College: Riverdale, NY, 1978–1984; Vols. 1–3.
2. Seebach, D.; Daum, H. Chem. Ber. 1974, 107, 1748; Schmidt,
M.; Anstutz, R.; Crass, G.; Seebach, D. Chem. Ber. 1980, 113,
1691.
3. Sih, C. J.; Chem, C. S. Angew. Chem. 1984, 96, 556.
4. Jones, J. B. Tetrahedron 1986, 42, 3349.
acid monoester (ꢀ)-8 (2.50 g, 8.98 mmol, Y: 25%), off white
25
crystals, {½aꢁD ¼ ꢀ2:7 (c 2, CHCl3)}.
5. Ohta, H.; Tetsukawa, H. Agric. Biol. Chem. 1980, 863.
6. Roberts, S. M.; Turner, N. J.; Willets, A. J.; Turner, M. K.
Introduction to Biocatalysis Using Enzymes and Microorgan-
isms; Cambridge University Press: New York, 1995.
7. Faber, K. Biotransformations in Organic Chemistry, 5th ed.;
Springer: Berlin, 2004.
8. Zaks, A.; Dodds, D. R. Drug Discovery Today 1997, 2, 513.
9. Stinson, S. C. Chem. Eng. News 1998, 76, 83.
10. (a) Ohno, M.; Otsaki, M. Org. React. 1990, 37, 1; (b) Wong,
C. H.; Whitesides, G. M. Enzymes in Synthetic Organic
Chemistry; Elsevier Science: Amsterdam, 1994; (c) Enzymes
Catalysis in Organic Synthesis—A Comprehensive Handbook,
2nd, Completely Revised and Enlarged ed.; Drauz, K.,
Waldmann, H., Eds.; VCH: Weinheim, 2002; (d) Santaniello,
E.; Ferraboschi, P.; Grisenti, P.; Manzocchi, A. Chem. Rev.
1992, 92, 1071; (e) Schoffers, E.; Golebiowski, A.; Johnson, C.
R. Tetrahedron 1996, 52, 3769.
The monoester was removed: (ꢀ)-1-benzo[1,3]dioxol-5-yl-
2-propanol maleic acid monoester (ꢀ)-8 (2.50 g) was
heated with a mixture of water (4 mL) and NaOH (1.2 g)
for 1 min and it was allowed to cool to room temperature.
The aqueous phase was washed with diethyl ether
(4 · 10 mL). The combined organic phase was dried over
Na2SO4 and the solvent was removed in vacuo to afford
(ꢀ)-1-benzo[1,3]dioxol-5-yl-2-propanol (ꢀ)-2 (1.46 g, 8.10
25
mmol, Y: 22.5%), oil, {½aꢁD ¼ ꢀ4:5 (c 2, CHCl3)}, ee: 14%.
The mother liquid was evaporated: (ꢀ)-1-benzo[1,3]dioxol-
5-yl-2-propanol maleic acid monoester (ꢀ)-8 (7.45 g,
25
26.77 mmol, Y: 74.5%), dun oil, {½aꢁD ¼ ꢀ12:7 (c 2,
CHCl3)}.
11. Oberhauser, T.; Faber, K.; Griengl, H. Tetrahedron 1989, 45,
1676.
12. Xe, Z.-F.; Suermune, H.; Sakai, K. Tetrahedron: Asymmetry
1990, 1, 395.
13. Eliel, E. L.; Wilen, S. H. Stereochemistry of Organic
Compounds; Wiley: New York, 1994.
14. Burgess, K.; Jennings, L. D. J. Am. Chem. Soc. 1991, 113,
6129–6139.
15. Cordo´va, A.; Trembley, M. R.; Clapman, B.; Janda, K. D. J.
Org. Chem. 2001, 66, 5645–5648.
16. Naemara, K.; Murata, M.; Tanaka, R.; Yano, M.; Hirose,
K.; Tobe, Y. Tetrahedron: Asymmetry 1996, 7, 3285–3294.
17. Suginaka, K.; Hayashi, Y.; Yamamoto, Y. Tetrahedron:
Asymmetry 1996, 7, 1153–1158.
18. Gutman, A. L.; Brenner, D.; Boltanski, A. Tetrahedron:
Asymmetry 1993, 4, 839–844.
19. Zmijewski, M. J.; Vicenzi, J.; Landen, B. E.; Muth, W.;
Marler, P.; Anderson, B. Appl. Microbiol. Biotechnol. 1997,
47, 162–166.
The monoester was removed: (ꢀ)-1-benzo[1,3]dioxol-5-yl-
2-propanol maleic acid monoester (ꢀ)-8 (7.45 g) was
heated with a mixture of water (11 mL) and NaOH
(3.8 g) for 1 min and it was allowed to cool to room tem-
perature. The aqueous phase was washed with diethyl ether
(4 · 10 mL). The combined organic phase was dried over
Na2SO4 and the solvent was removed in vacuo to afford
(ꢀ)-1-benzo[1,3]dioxol-5-yl-2-propanol
(ꢀ)-2
(4.24 g,
25
23.53 mmol, Y: 65.5%), oil, ½aꢁD ¼ ꢀ19:1 (c 2, CHCl3),
ee: 59%.
4.15.2. Purification of (ꢀ)-1-(3,4-dichloro-phenyl)-2-propa-
nol (ꢀ)-5 enantiomer with recrystallization: general proce-
dure. (ꢀ)-1-(3,4-Dichloro-phenyl)-2-propanol
(ꢀ)-5
(6.75 g, 32.91 mmol, ee: 61%) was dissolved in hot hexane
(6.8 mL). The solution was divided into two phases during
the cooling to 20 ꢁC. The two-phase solution was inocu-
lated with pure (ꢀ)-1-(3,4-dichloro-phenyl)-2-propanol
(ꢀ)-5 and it was stirred for 20 min. The crystals were
20. Anderson, B. A.; Hansen, M. M.; Harkness, A. R.; Henry, C.
L.; Vicenzi, J. T.; Zmijewski, M. J. J. Am. Chem. Soc. 1995,
117, 12358–12359.