Organic and Biomolecular Chemistry p. 1443 - 1446 (2003)
Update date:2022-07-30
Topics:
Goodwin, Catherine E.
Leeper, Finian J.
Several (3R)- and (3S)-Deuterated forms of 5-aminolaevulinic acid were synthesized. The resultant (3R)-form shows a significantly larger isotope effect when incubated with porphobilinogen synthase from bovine liver and from Bacillus subtilis. Based on the results of this study and on available crystal structures, a modified mechanism for the enzyme reaction was established.
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