Isolation and characterization of new bufotoxins from the skin of Bufo melanostictus Schneider

Article abstract of DOI:10.1248/cpb.32.4396

The occurrence of new bufotoxins, in which L-histidine, L-3-methylhistidine, or L-1-methylhistidine replaces the arginine residue of hitherto known 'bufotoxin', in the skin of the Formosan toad, Bufo melanostrictus SCHNEIDER, is reported. Five novel bufotoxins, bufotalin 3-suberoyl-L-histidine, -3-methylhistidine and -1-methylhistidine esters and 19-hydroxybufalin 3-suberoyl-L-histidine and -3-methylhistidine esters, were separated by usual chromatographic methods including reversed phase high-performance liquid chromatography, and their structures were elucidated by degradative and synthetic means.