Turn-on detection of targeted biochemical reactions by triple resonance NMR analysis using isotope-labeled probe

Article abstract of DOI:10.1246/cl.2010.926

We report on a strategy for the "turn-on" detection of target biochemical (metabolic) reactions using a triple resonance NMR technique with an isotope-labeled probe. Our NMR study clearly reveals that otherwise NMR-nonactive-^l3C/²H-labeled glucose actually turns "on" its ¹HNMR signal by conversion to an important biomarker lactate as the end product of anaerobic glycolysis in cells and in injected mice with high selectivity.