Organic and Biomolecular Chemistry p. 2267 - 2275 (2003)
Update date:2022-08-05
Topics:
McKeen, Catherine M.
Brown, Lynda J.
Nicol, Jamie T.G.
Mellor, John M.
Brown, Tom
The synthesis of monomers to incorporate fluorescein and methyl red within sequence and at the termini of modified oligonucleotides was reported. These monomers were used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures. Fluorescence quenching and energy transfer were also used to study the structural, thermodynamic and kinetic properties of higher order nucleic acid structures.
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