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using OMFP as a substrate in 62.5 mM MOPS buffer,
pH 7.0, containing 62.5 mM NaCl, 0.125 mg/mL BSA,
0.0125% Brij35, 1.25 mM EDTA and 1.25 mM DTT. The
reactions were initiated by the addition of enzyme and
quenched by the addition of stop solution (0.25 N NaOH).
Fluorescence emissions from the product o-methyl fluo-
rescein were measured using an appropriate plate reader
and converted to units of product formation. IC50 values
were calculated by nonlinear regression using the dose-
response equation in GraphPad Prism. For inhibitor
kinetics, best curve fit for Lineweaver–Burk plots and Ki
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reference compounds, which gave IC50 and Ki values that
were consistent with reported values. In some experiments,
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of p-nitrophenyl phosphate.
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