Facile amide bond formation from esters of amino acids and peptides catalyzed by alkaline protease in anhydrous tert-butyl alcohol using ammonium chloride/triethylamine as a source of nucleophilic ammonia

Article abstract of DOI:10.1055/s-1993-25955

An industrial alkaline protease 'Alcalase', stable and active in tert-butyl alcohol, was used to catalyze the synthesis of N-protected amino acids or peptide amides in anhydrous tert-butyl alcohol using ammonium chloride/triethylamine as source of nucleophilic ammonia