Nucleosides and Nucleotides p. 815 - 829 (1998)
Update date:2022-07-29
Topics:
Nawrot
Sprinzl
Reaction of isatoic anhydride with adenosine, adenosine 5'-phosphate, oligoribonucleotides or with the E. coli tRNA(Val) led to attachment of an anthraniloyl residue at 2'- or 3'-OH groups of 3'-terminal ribose residue. No protection of the 5'-hydroxyl group or internal T-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl- residue is used as a fluorescent reporter group to monitor interactions with proteins.
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