Aminoacyl-tRNA analogues; synthesis, purification and properties of 3'- anthraniloyl oligoribonucleotides

Article abstract of DOI:10.1080/07328319808004677

Reaction of isatoic anhydride with adenosine, adenosine 5'-phosphate, oligoribonucleotides or with the E. coli tRNA(Val) led to attachment of an anthraniloyl residue at 2'- or 3'-OH groups of 3'-terminal ribose residue. No protection of the 5'-hydroxyl group or internal T-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl- residue is used as a fluorescent reporter group to monitor interactions with proteins.

Full text of DOI:10.1080/07328319808004677

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Aminoacyl-tRNA Analogues; Synthesis,
Purification and Properties of 3′-
Anthraniloyl Oligoribonucleotides
B. Nawrot a & M. Sprinzl a
a Laboratorium für Biochemie, Universität Bayreuth, D-95440,
Bayreuth, Germany
Published online: 13 Feb 2011.
To cite this article: B. Nawrot & M. Sprinzl (1998) Aminoacyl-tRNA Analogues; Synthesis, Purification
and Properties of 3′-Anthraniloyl Oligoribonucleotides, Nucleosides and Nucleotides, 17:4, 815-829,
DOI: 10.1080/07328319808004677
To link to this article: http://dx.doi.org/10.1080/07328319808004677
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