SYNTHESIS OF OLIGODEOXYNUCLEOTIDES AND OLIGODEOXYNUCLEOTIDE ANALOGS USING PHOSPHORAMIDITE INTERMEDIATES

Article abstract of DOI:10.1016/0040-4020(84)85107-8

The synthesis of two deoxyoligonucleotides, d(G-T-G-A-G-T-T-A-G-C-T-C-A-C) and d(G-T-G-A-G-C-T-A-A-C-T-C-A-C), corresponding to the DNA binding site for cyclic AMP receptor protein is reported.These syntheses have been completed in milligram quantities using a silica gel polymer support methodology and mononucleotide phosphoramidites.Procedures are also reported for synthesizing diastereoisomers of dinucleoside methylphosphonate 3'-phosphoramidites.