A chemical model for the enzymatic mono de-alkylation of (methyl and ethyl) parathion by glutathione-S-transferase

Article abstract of DOI:10.1016/s0040-4020(97)10176-4

The NMR study of the reaction of methylparathion with thiol (glutathione) and a tertiary amine shows unambiguously a sequential transfer of the methyl group firstly to the amine and secondly to the thiol. The reaction stops after a single demethylation. This observation may account for regiospecificity of some glutathione S-transferases.