Enzyme-Catalyzed Organic Synthesis: NAD(P)H Cofactor Regeneration by Using Glucose 6-Phosphate and the Glucose-6-Phosphate Dehydrogenase from Leuconostoc mesenteroides

Article abstract of DOI:10.1021/ja00406a037

Glucose 6-phosphate dehydrogenase (from Leuconostoc mesenteroides) and glucose 6-phosphate comprise a useful system for regeneration of reduced nicotinamide cofactors for use in enzyme-catalyzed organic sysnthesis.This enzyme is approximately equally active in reduction of NAD and NADP.It is commercially available, inexpensive, stable, and easily immobilized.Glucose 6-phosphate can be prepared in quantity by hexokinase-catalysed phosphorylation of glucose by ATP (coupled with ATP regeneration) or by other methods.The operation in this regeneration system is illustrated by syntheses of enantiomerically enriched D-lactic acid (0.4 mol, enantiomeric excess 95percent) and (S)-benzyl-α-d1 alcohol (0.4 mol, enantiomeric excess 95percent) and by synthesis of treo-D_S(+)-isocitric acid (0.17 mol).Factors influencing the stability of NAD(P)(H) in solution have been explored.