Separation of Amino Acid Enantiomers and Chiral Amines Using Precolumn Derivatization with (+)-1-(9-Fluorenyl)ethyl Chloroformate and Reversed-Phase Liquid Chromatography

Article abstract of DOI:10.1021/ac00135a025

A new chiral reagent (+)-1-(9-fluorenyl)ethyl chloroformate (FLEC) was synthesized for separation of amino acid enentiomers and optically active amines.Highly fluorescent diastereomers of amino acids were obtained without racemization within 4 min at room temperature.The derivatives have favorable chromatographic properties, which is demonstrated by a reversed-phase separation of the D and L form of 17 primary amino acids in a single run.The secondary amino acids were resolved separately at a lower pH and can be determined without interferences from primary amino acids.The stability of the derivatives permitted confirmation studies with gas chromatography-mass spectrometry.