Journal of labelled compounds and radiopharmaceuticals p. 221 - 226 (1998)
Update date:2022-08-11
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Tait
[3α-3h]-dehydroepiandrosterone ([3α-3h]-3β-hydroxy-5-androsten-17- one) and [3α-3h]-pregnenolone ([3α-3h]-3β-hydroxy-5-pregnen-20-one) were prepared by selective reduction of 3-keto-5-ene intermediates with tritiated sodium borohydride. These were used as substrates to set up a tritium release assay for 3β-hydroxysteroid oxido-reductase and 5→4-ene isomerase (3β- HSD) which is a key enzyme in steroidogenesis.
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