RSC Advances
Paper
2
0 mL (Fig. S8†). The HPLC conditions for analyzing other
amino acids were: mobile phase A 5% acetonitrile (0.05% tri-
uoroacetic acid, 1% methanol), mobile phase B 60% acetoni-
trile (0.05% triuoroacetic acid, 1% methanol), linear gradient
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ꢀ1
from 0% B to 100% B over 45 min at a ow rate 1 mL min
injection volume 20 mL (Fig. S9–S16†).
,
47
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Preparation, isolation, and characterization of D-Phe
ꢀ1
In a 50 mL reaction system, 80 mM L-Phe, 40 mg mL PmLAAD
ꢀ
1
ꢀ1
whole cells, 4.5 mg mL
StDAPDH/H227V, 0.35 mg mL
+
BsFDH, 8 mM NADP , 240 mM NH
4
Cl, and 160 mM sodium
formate were added in the reaction buffer (50 mM Tris–HCl, pH
ꢁ
9
.0). Reactions were carried out at 220 rpm and 45 C. Aer 6 h,
pH of the reaction mixture was adjusted by diluted HCl and the
precipitated enzymes were removed by centrifugation. The
product was isolated by a cation-exchange resin (732) column
4
3, 788–824.
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1
1
1
1
1
1
20
3 2
and eluted by 6% NH $H O. The product was characterized by
4
1
13
1
MS, H-NMR and C-NMR as follows (Fig. S5–S7†): H-NMR
400 MHz, D O), d/ppm: 3.11 (m, 1H), 3.28 (m, 1H), 3.98 (m,
1 Organic Synthesis Using Biocatalysis, ed. S. K. Au, J. Groover,
B. D. Feske and A. S. Bommarius, Elsevier, 2016.
(
1
5
2
1
3
H), 7.38 (m, 5H); C-NMR (100 MHz, D
6.03, 129.11, 129.37, 135.11, 173.89.
2
O), d/ppm: 36.34,
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Conflicts of interest
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There are no conicts of interest to declare.
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3
509.
16 R. Mor ´a n-Ramallal, R. Liz and V. Gotor, Org. Lett., 2008, 10,
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Acknowledgements
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This work was nancially supported by the National Natural
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29934 | RSC Adv., 2019, 9, 29927–29935
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