Potential D,L-amino acid sequence analysis of peptides from the C-terminus

Article abstract of DOI:10.1271/bbb.61.392

A model tripeptide, Gly-L-Leu-L-Phe, was immobilized with activated aminomethyl polystyrene, and its C-terminal was reduced to an alcohol. This peptidyl alcohol was selectively hydrolyzed at the C-terminal amide bond to afford a polymer-supported dipeptide (Gly-L-Leu) and amino alcohol (Phe-OH). The amino alcohol, including its absolute configuration, was determined by labelling with (+)-MNB-COOH, and the dipeptide was reused for a determination of its C-terminal amino acids. The D,L-amino acids of the tripeptide were sequentially determined from the C-terminus.

Full text of DOI:10.1271/bbb.61.392

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Potential d,l-Amino Acid Sequence Analysis of
Peptides from the C-Terminus
Hiroshi Ohruia, Eigo Itoha, Yoshihiro Nishidaa, Hiroko Horiea & Hiroshi Meguroa
a Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University,
Tsutsumidori-Amamiyamachi 1–1, Aoba-ku, Sendai 981, Japan
Published online: 12 Jun 2014.
To cite this article: Hiroshi Ohrui, Eigo Itoh, Yoshihiro Nishida, Hiroko Horie & Hiroshi Meguro (1997) Potential d,l-Amino
Acid Sequence Analysis of Peptides from the C-Terminus, Bioscience, Biotechnology, and Biochemistry, 61:2, 392-395, DOI:
10.1271/bbb.61.392
To link to this article: http://dx.doi.org/10.1271/bbb.61.392
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