Synthesis, spectral and biological studies of complexes with bidentate azodye ligand derived from resorcinol and 1-amino-2-naphthol-4-sulphonic acid

Article abstract of DOI:10.13005/ojc/340105

A new series of transition metal complexes of Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), and Fe(III) with the azo dye, 3-hydroxy-4-(2,4-dihydroxyphenylazo)naphthalene-1-sulphonic acid (LH) have been synthesized and characterized. The structural features have been arrived from their microanalysis, magnetic susceptibility, molar conductance measurements, mass, fluorescence, IR, UV-Vis, ¹HNMR and ESR spectral studies. M?ssbauer spectra of two iron complexes have been done. In the light of these results, it is suggested that ligand coordinates to metal ions via hydroxyl oxygen and nitrogen of azo group. The fluorescence spectral property of ligand and complexes studied where complexes shows enhanced emission. The redox property of the Co(II) complex is studied by cyclic voltammetry. The ligand and its Mn(II) complex was investigated for non linear optical property. The in vitro biological activity of the ligand and complexes were tested against Escherichia coli, Staphylococcus aureus, Aspergillus Niger and Candida albicans. The results indicate that the complexes have enhanced biological activity than the ligand. Antioxidant activity of the ligand and complexes was studied by free radical scavenging method. The nuclease activity of ligand and complexes show that they cleave DNA through redox chemistry.

Full text of DOI:10.13005/ojc/340105

ISSN: 0970-020 X
CODEN: OJCHEG
018, Vol. 34, No.(1):
Pg. 45-54
ORIENTAL JOURNAL OF CHEMISTRY
An International Open Free Access, Peer Reviewed Research Journal
2
www.orientjchem.org
Synthesis, Spectral and Biological Studies of Complexes
with Bidentate Azodye Ligand Derived from Resorcinol and
1
-amino-2-naphthol-4-sulphonic Acid
1
1
2
2
V. G. VIDYA *,V. SADASIVAN , S. S. MEENA and PRAMOD BHATT
1
University College, M G Road, Palayam, Thiruvananthapuram- 695 034, Kerala, India.
2
Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
*
Corresponding author E-mail: vg_vidya@yahoo.co.in
http://dx.doi.org/10.13005/ojc/340105
(Received: August 30, 2017; Accepted: September 15, 2017)
ABSTRACT
A new series of transition metal complexes of Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II),
and Fe(III) with the azo dye, 3-hydroxy-4-(2,4-dihydroxyphenylazo)naphthalene-1-sulphonic acid
(LH) have been synthesized and characterized. The structural features have been arrived from
their microanalysis, magnetic susceptibility, molar conductance measurements, mass,
1
fluorescence, IR, UV-Vis, HNMR and ESR spectral studies. Mössbauer spectra of two iron
complexes have been done. In the light of these results, it is suggested that ligand coordinates to
metal ions via hydroxyl oxygen and nitrogen of azo group. The fluorescence spectral property of
ligand and complexes studied where complexes shows enhanced emission. The redox property
of the Co(II) complex is studied by cyclic voltammetry. The ligand and its Mn(II) complex was
investigated for non linear optical property. The in vitro biological activity of the ligand and complexes
were tested against Escherichia coli, Staphylococcus aureus, Aspergillus niger and Candida
albicans. The results indicate that the complexes have enhanced biological activity than the
ligand. Antioxidant activity of the ligand and complexes was studied by free radical scavenging
method. The nuclease activity of ligand and complexes show that they cleave DNA through redox
chemistry.
Keywords: Azo dye, Mössbauer spectra, NLO property, DNA cleavage, Antioxidant activity.
INTRODUCTION
of the transition metals that plays an important role
in the development of coordination chemistry .
2
In the last few years, a lot of interest have
Azo dyes have massive applications in various
fields of science and technology they serve as a
class of widely used colouring materials .They play
been taken by chemists in the synthesis and
physicochemical studies of first row transition metal
3
1
complexes with a number of azo dye ligands .
a major role in textile, printing, leather, paper
4
5
Azo dyes readily form stable complexes with most
making, drugs and in food industries . The
This is an
.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/ ), which permits unrestricted
NonCommercial use, distribution and reproduction in any medium, provided the original work is properly cited.
Open Access article licensed under a Creative Commons Attribution-NonCommercial-ShareAlike
4

4
6
VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
increasing use of these dyes in electronic industry Preparation of 3–hydroxyl-4-(2,4dihydroxy
such as colorimetric sensors, non linear optical phenylazo)naphthalene-1-sulphonic acid [LH]
(NLO) devices and liquid crystalline displays. They
A solution of 1-amino-2-naphthol-4-
are also used as potential sensitizers for sulphonic acid (0.01 mol) was prepared in least
6
o
photodynamic therapy . In bioinorganic chemistry, amount of HCl and cooled to 0 C. A cold solution of
o
interest in azo dye complexes has centered on the NaNO below 5 C was added to the amine solution
2
role that complexes play in providing synthetic drop wise with continuous stirring. This cold
models for the metal containing sites in metallo- diazonium salt solution was added to resorcinol
7
,8
o
proteins and enzymes . Metal ions involved in solution (0.01 mol in NaOH) kept at 0 C drop wise
1
1
biological processes of life has been a subject of with stirring . The dye separated was filtered off
interest . The modes of action of metal ions are and dried in vacuo over anhydrous CaCl .The purity
9
,10
2
often complex but are believed to involve bonding of azo dye was confirmed by TLC.
with the hetero atoms of the heterocyclic residues
of biological molecules.
Preparation of the complexes
The complexes of LH were prepared by
In the present paper, the synthesis and mixing ethanolic solutions of respective metal salt
characterization of a new azo dye and its complexes and LH in molar ratio 1:2. The solution obtained
have been reported. The aim of the study is to was heated under reflux for 4 h for completion of
understand the spectrochemical and biological the reaction. The solid compound deposited filtered
activities of this new dye and its complexes.
and washed three times with ethanol and dried over
silica gel in a desiccator.
EXPERIMENTAL
RESULTS AND DISCUSSION
All reagents and solvents for synthesis
and analysis were Merck products and used as
The metal and chloride content of the
supplied. FTIR spectra were recorded using KBr reported complexes were estimated following
12
discs on a Perkin-Elmer Spectrum 65 spectrometer. standard procedure . Analytical data of complexes
Elemental analyses performed on aVario EL-III CHN and the ligand are presented in Table.1. The
elemental analyses shows that complexes has
analyser. Conductivity measurements were made
using Systronics direct reading conductivity meter.
The magnetic susceptibilities were measured on a
magnetic susceptibility balance Sherwood
Scientific. Electronic absorption spectral
measurements of the ligand and the complexes in
methanol were conducted using Perkin-Elmer
Lambda 25 UV-Vis spectrophotometer. H NMR
spectra was recorded on a Bruker Avance III,
4
general stoichiometry of type [ML (H O) ] where
2
2
2
M = Mn(II), Co(II), Ni(II), Fe(II), Cu(II), [ZnL ] and
2
[
FeL Cl(H O)].
2
2
The ligand and its metal complexes are
air stable, soluble in DMSO and DMF.
1
1
The H-NMR spectra of LH and its Zn(II)
complex were recorded in d6-DMSO solution on a
00MHz FTNMR instrument usingTMS as reference. 400 MHz FTNMR spectrometer using TMS as the
1
The fluorescence spectral measurements of ligand and reference material. The H-NMR of LH shows a
complexes in methanol were recorded on a JASCO- multiplet betweenδ 6.1 - 7.7 ppm (8H,m) due to aryl
protons.The peaks respectively at δ 8.7 ppm (1H,s),
δ 11.1(1H,s) and δ 9.3(1H,s) are assignable to OH
of resorcinol and naphthol group. The singlet
observed at δ 11.1 ppm due to one − group in LH is
absent in the Zn(II) complex indicating
deprotonation of one hydroxyl group before
coordination to metal ion. The two other − protons
were shifted to lower field.This indicates that among
three phenolic − group one gets deprotonated and
7
50 fluorescence spectrometer. The Mössbauer
spectra of iron complexes recorded using
Mossbauer Spectrometer Nucleonix Systems,
constant acceleration mode at room temperature.
The source employed was Co-57 in Rh matrix of
strength 50 mCi. The velocity scale calibration was
5
7
done using an enriched α Fe metal foil of line
width 0.23 mm/s using a Win-Normos fit program.
The voltammetric measurements were carried on a coordinated to Zn(II) and others remain as such.
BAS CV-50W voltammetric analyzer. The second The values of aryl protons were also shifted to lower
harmonic generation (SHG) conversion efficiency field indicating chelation of ligand. The singlet at δ
of ligand determined by modified version of powder 2.51 ppm corresponds to proton of sulfonic acid
13
technique.
group in the LH shifted to lower field of δ 2.72 ppm .

VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
47
1
The H-NMR spectrum of LH and [ZnL ] is given in
at m/z 784 and (M+Na) peak at m/z 806 confirms its
2
stoichiometry. Mass spectrum of [ZnL ] is shown in
Figure. 4.
Figure. 1 and Figure.2.
2
The ESI mass spectra of the LH shows a
The molar conductance of the complexes
measured in methanol, DMF and in nitrobenzene
supports the non electrolytic nature.
molecular ion peak at m/z =361 shown in Fig. 3.
The mass spectrum of [ZnL ] shows a (M + 1) peak
2
1
Fig.1. H-NMR spectra of [LH]
1
Fig 2. H-NMR spectra of [ZnL ]
2
Azo dyes are not only mere dyestuffs but
also used in photodynamic therapy.There are recent
reports on the photoluminescence properties of azo
dyes, which are potential systems for photodynamic
14
therapy . LH, [MnL (H O) ] and [NiL (H O) ] exhibit
2
2
2
2
2
2
fluorescence in the range 350-700 nm in methanol
solution at room temperature. These are assigned
Fig 3. Mass spectra of [LH]

4
8
VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
The -N=N- stretching vibration of LH at
-1
-1
1459 cm shifted to 1431-1438 cm in its complexes
indicating the azo group participation in
coordination. All the complexes show additional
-1
band around 416 - 432 cm due to M-N coordination.
The Stretching in LH complexes appears
-
1
-1
-1
respectively at 3429 cm , 3436 cm and 3420 cm
as broad band is due to hydrogen bonded hydroxyl
groups. In the IR spectra of the complexes the
-
1
disappearance of the band at 3420 cm indicates
deprotonation of one OH group. This infers that one
OH gets coordinated after deprotonation and others
remain as such. The appearance of a new broad
Fig 4. Mass spectra of [ZnL2]
-
1
as intraligand (π→π∗) fluorescence. It is interesting band around 3443 cm in complexes indicates the
that Mn(II) and Ni(II) complexes show enhanced presence of water molecule. The presence of
emission than that of free ligand. Metal ions can coordinated water molecule is again confirmed by
enhance the fluorescence emission of azo dye.The the presence of two other bands within the ranges
-
1
fluorescence spectra of LH, [MnL (H O) ] and 1630-1600 and 970-950 cm due to HOH
2
2
2
[
NiL (H O) ] are given in Fig 5. LH shows two deformation and rocking. A band observed in range
2 2 2 .
-
1
emission bands at 470 and 594 nm when excited at 1201 -1216 cm is due to the symmetric stretching
20 nm. [MnL (H O) ] shows emission at 407 nm vibration of -SO H group. The appearance of new
3
2
2
2
3
-1
-1
and [NiL (H O) ] shows two bands at 409 and 569 bands in the region 510-523 cm and 415-435 cm due
2
2
2
nm when excited at 353 and 360 nm respectively.
to ν M-O and ν M-N supports the coordination of OH
15
group and -N=N- group to metal ions . The IR
The infrared spectra of LH and its metal spectral evidences discussed so far indicate that
complexes were recorded in the range the ligand is coordinated to the metal ion in
-
1
4
00 – 4000 cm . The infrared spectra of the uninegative bidentate manner. IR spectral data of
complexes show characteristic absorptions of all LH and complexes are given in Table. 2.
functional groups of the ligand but at shifted position
in the case of coordinated groups of the ligand.
The electronic spectra of ligand shows two
absorption maxima at 320nm and 340 nm
corresponding to n→π∗ and π→π* transitions and
undergoes bathochromic shift upon coordination.
The spectrum of Cu(II) complex shows a
2
2
shoulder in 462 nm due to A → B d-d transition.
1
g
1g
The observed magnetic moment of this complex is
.38 BM.This subnormal effective magnetic moment
value indicates that the copper centres are
1
1
6
ferromagnetically coupled . The Ni(II) complex
exhibits weak bands at 520 nm and 588 nm
3
3
3
3
Fig. 5. Photoluminescence spectra of [LH]
corresponds to A (F)→ T (P) and A (F)→ T
2g 1g 2g 1g
-
1
Table 2. Selected FT-IR data for ligand and its complexes (cm )
3
-
1
-
420
459
-
-
-
-
-
-
-
-
νOH
ν H2O
νN=N
νM-O
νM-N
3447
1435
515
417
3446
1431
510
416
3447
1438
523
432
3445
1438
517
418
3444
1435
518
420
3434
1432
520
422
1433
518
418
-

VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
49
(
F) transitions. The magnetic moment of this
misaligned. The g values are taken to calculate the
exchange interaction, if G > 4, the exchange
coupling is negligible .
complex is 2.83 BM suggesting an octahedrally
coordinate Ni(II) complex.The Co(II) complex exhibit
peaks at 477 nm and 675 nm for T (F)→ T (P)
and T1g (F) → A (F) transitions. The magnetic
moment value of 4.36 BM also indicates an
octahedral geometry for the complex. The Fe(III)
complex exhibits band at 660 nm and 680 nm
1
9
4
4
1g
1g
Table. 3: Electron Spin Resonance parameters
of copper complex
4
4
2g
2
Complex
gll
g
⊥
g avg
All
G
α
6
4
6
4
assigned to A1g → T and A1g → T transitions.
2g
1g
[
CuL2]
2.41 2.06 2.17 140 7.11 0.86
The magnetic moment value of 5.71 BM agrees to
the octahedral high spin geometry of complex. The
Fe(II) complex exhibit bands at 510 nm and 660
The cyclic voltammogram of the Co
5
5
nm due to T → E transitions. This complex has
complex in MeCN in potential range 1.5 to -1.5 V
shows a well defined redox process corresponding
to the formation of Co(II)/Co(I) couple. A reduction
peak at Epa = - 0.39 V and at Epc = -0.656 V. This
2
g
g
an octahedral high spin state with a magnetic
moment value of 5.52 BM. The Mn(II) exhibits a
band at 474 nm due to d-d transition.The magnetic
moment is 4.84 BM suggesting a distorted octahedral
couple is found to be reversible with ΔE = 0.266V.
p
18
geometry .
The ratio of anodic to cathodic peak currents (I / I
pa pc
=
1) corresponding to one electron process. The
The X-band ESR spectrum of copper
complex was recorded at 77 K is shown in Fig. 6.
The frozen solution shows a well resolved four line
hyperfine splitting in parallel region corresponds
to the electron spin –nuclear spin interaction. The
absence of half field signal at 1600G, corresponding
to ΔM = ±2 transition, rules out any Cu-Cu
s
interaction in ESR spectra. The frozen solution
shows a well resolved four line hyperfine splitting
in parallel region corresponding to the electron
spin –nuclear spin interaction. The Cu complex
exhibits two different g values, indicating the
magnetic anisotropy in the complex. It exhibits two
different g values, indicating the magnetic
anisotropy in the complex. The spin Hamiltonian
parameters, calculated for the complex are given
inTable.3.The g tensor values of the Cu(II) complex
can be used to derive the ground state.
Fig. 6. ESR spectra of [CuL2]
complex shows another quasi reversible peak in
the anodic region, characteristics of Co (II) / Co(III)
couple at E = 0.219V and at E = 0.196V for Co(II)
pc
pa
/
Co(III) oxidation with ΔE 0.023V.The voltammetric
p
The g (2.41) > g
⊥
(2.06) > g (2.0027)
studies proved that these complexes are relatively
more stable. The peak separation of this couple is
0.023 V. The ratio of anodic to cathodic peak
currentsH”1 corresponding to one electron process.
The tapering of peak both in cathodic and anodic
ll
e
indicates that the complex has an axially elongated
octahedral geometry. Further it is supported from
the fact that the unpaired electron lies
predominantly in dx2-y2 orbital as was evident from
the value of exchange interaction term, G. The
perpendicular component shows no hyperfine
21
side may be due to ligand oxidation . The cyclic
voltammogram of [CoL (H O) ] is shown in Figure. 7.
2
2
2
2
splitting A⊥ ≈ “=” 0. The covalency factor α
calculated for the complex is 0.86, less than one
indicates that the complex has some covalent
character in ligand environment.
The Mössbauer spectra of iron complexes
were recorded at room temperature and given in
Fig. 8 and 9 and Mössbauer parameters in Table. 4
If G > 4, local tetragonal axes are aligned
parallel or only slightly misaligned. The observed
value for exchange interaction parameter for the
Cu complex (G = 7.11) suggests that the local
tetragonal axes are aligned parallel or slightly
The [FeL Cl(H O)] gives a signal with an
2
2
isomer shift δ=0.35 mm/s.The observed isomer shift
value suggests that Fe(III) nucleus is in high spin
state. A single signal indicates presence of some
22
symmetry around Fe(III) ion in complex .

5
0
VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
[
FeL (H O) ] shows four types of doublet around the iron atom. The values are consistent of
2
2
2
with isomer shift value of 1.48, 1.26, 0.23 and 0.40 iron (II) high spin configuration and are in agreement
2
3,24, 25,26,27,28
mm/s and quadruple splitting of 2.74, 3.29, 1.07 with magnetic moment value 4.52 BM
and 1.15 mm/s respectively indicating that there are
.
four different species present in complex.The above
The LH (L3), [MnL (H O) ] (M3), [NiL
2 2 2 2
parameters are characteristic of octahedrally (H O) ] (N3) and [ZnL ] (Z3) complexes were tested
2
2
2
coordinated high spin Fe(II) complex. The isomer for their inhibitory activity on antimicrobial growth
shift does not indicate a similar electron density by a modified disc diffusion method against
Fig. 7. CV of [CoL (H O) ]
2
2
2
Escherichia coli, Staphylococcus aureus, concentration (IC50 in μg/ml) shown in Fig.11. IC50
Aspergillus niger and Candida albicans. The
standard drugs clotrimazole and gentamycin were
taken as the standard. From the results it is clear
that the inhibition zone of the complex is higher
than that of the ligand. The higher activity of
values of LH , [MnL (H O) ] (M3) and [NiL (H O) ]
2
2
2
2
2
2
(N3) are 187±0.25, 166±0.13, and 185±0.12 μg/ml
respectively. Mn complex shows better inhibitory
30
action than ligand .
2
9
complexes can be explained by chelation . The
results are given in Figure.10.
The DNA gel electrophoresis experiment
was conducted at room temperature using ligand
and complexes in presence of H O as oxidant
The antioxidant activities of these
2
2
compounds are expressed as 50% inhibitory (Fig.12). Both ligand (L3) and its Ni(II) (N3) and Mn(II)
Fig. 8. Mössbauer spectra of [FeL Cl(H O)]
2
2

VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
51
Fig. 9. Mössbauer spectra of [FeL (H O) ]
2
2
2
Table. 4: Mössbauer parameters of compounds [FeL Cl(H O)]and [FeL (H O) ]
2
2
2
2
2
a
b
c
d
r
Compound
Spectral l; km IS, (mm/s) QS, (mm/s) Fe oxidation
FWHM
(mm/s)
S ,(%)
component
state
[
FeL Cl(H O)]
Signal
+0.356
+1.480
+1.26
+0.203
+0.40
-
III(HS)
II(HS)
II(HS)
II(LS)
II(LS)
1.49±0.402
0.44±0.094
0.21±0.017
0.55±0.118
0.22±0.040
100.0
16.00
36.13
25.69
22.12
2
2
[FeL (H O) ]
Doublet I
Doublet II
Doublet III
Doublet IV
2.740
3.29
1.072
1.151
2
2
2
a
b
c
Comp.: Compound, Isomer Shift (IS) (relative to á-Fe). Quadruple splitting (QS). Full line width at half
d
maximum (FWHM), Partial resonant absorption areas (R ) of spectral components which represent
A
relative contents of the corresponding form assuming a common recoilless fraction for all forms. HS: High
spin, LS: Low spin.
M3 complexes exhibit nuclease activity due to their
efficient DNA binding ability.The ligand and
complexes were able to convert supercoiled DNA
into open circular DNA. The reaction is modulated
Control does not show any cleavage. This may be
due to formation of redox couple of metal ions and
its behavior. Further the presence of smear in the
gel diagram indicates the presence of radical
3
1
32
by metallocomplex bound hydroxyl radical .
cleavage .
Fig.10. Antimicrobial activity of LH and its complexes

5
2
VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
The Co(II) complex has been tested for
cytotoxic activity against two human cancer cell line
The SHG efficiency of ligand and complex
done by modified version of powder technique by
SiHa and SkBr .The cells revived with RPMI medium
35
3
Kurtz and Perry .The second harmonic generation
containing 20% FBS. Procedure done in laminar efficiency of LH and its [MnL (H O) ] complex were
2
2
2
air flow. For sub culturing, cells are given a media determined.The efficiency of sample was compared
change a day after they are revived. Then onwards with microcrystalline powder of KDP. LH shows 5.1
SHG/mV, [MnL (H O) ] shows 5.3 SHG/mV
RPMI containing 10% FBS is used. Then cells are
seeded by transferring them onto 96 well plates for
Hoechst staining. The drugs were added after cells
attain around 60% confluency in the culture plate
and apoptosis was induced in them and observed
under fluorescent microscope and is given in Fig.13.
It is interesting to observe that 100% chromatin
2
2
2
compared to 11 SHG/mV for KDP. The percentage
efficiency of LH is 46.36% and for [MnL (H O) ] it is
2
2
2
48.18% with respect to KDP. Mn(II) complex shows
enhanced activity compared to LH.The experimental
data infers that ligand and complex are less active.
Though the present investigated azodye and its
complex possesses a pathway of conjugated electrons
condensation has occurred with SkBr and an the NLO activity is low and this may be due to absence
3
average of 95.5% condensation happened in SiHa of electron pushing and pulling substituents on aromatic
3
2, 33,34
36
compared to that of control
.
rings .
Fig. 11. Antioxidant activity
Fig. 12. DNA cleavage
CONCLUSION
Fig. 13. Cytotoxic activity
data shows that in all complexes the metal ligand
ratio are 1:2. The conductance value suggest that
The coordination chemistry of ligand all complexes are non-electrolyte in nature. The
-hydroxy-4-(2,4-dihydroxyphenylazo)naphthalene- hydroxyl proton of LH appeared at 11.10 ppm is
-sulphonic acid has been investigated Analytical absent in Zn(II) complex, which supports the
3
1

VIDYA V G et al., Orient. J. Chem., Vol. 34(1), 45-54 (2018)
53
deprotonation of one OH group. The other NMR
signals are shifted to lower field indicating the
ligand chelation. The mass spectrum of LH and
Zn(II) complex agrees well with the analytical data
obtained. The infrared spectra of complexes
indicates that LH acts as univalent bidentate ligand.
Infrared spectral data also support the presence of
coordinated water molecule in all complexes except
for Zn(II). The mass spectrum supports this
observation. The coordination centers are azo
nitrogen and phenolic oxygen after deprotonation.
All complexes exhibit octahedral geometry except
for Zn(II) complex which is tetrahedral.This is further
evidenced by electronic spectra and magnetic
moment values. . IR spectral data suggest univalent
bidentate chelation of ligand and coordination sites
are azo nitrogen and phenolic OH after
deprotonation. The Mn(II) and Ni(II) complexes
show enhanced fluorescence. The NLO activity of
Mn(II) complex is comparatively higher compared
to the ligand. The metal complexes have enhanced
antimicrobial activity than ligand. Antioxidant
property of the ligand and complexes also studied.
The interaction of ligand and complexes with DNA
was investigated by gel electrophoresis. It is
observed that ligand and complexes cleave DNA
as compared to control in the presence of H O .
2
2
This report has also highlighted the importance of
Co(II) complex for anticancer activity.
ACKNOWLEDGMENT
The authors are thankful to RGCB,
Trivandrum, for cytotoxic studies and Department
of Chemistry, University College, Trivandrum for
instrumental facilities under DST FIST. I author is
thankful to UGC for the FDP support.
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