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Journal of Materials Chemistry B
Page 5 of 6
DOI: 10.1039/C7TB00379J
Journal Name
ARTICLE
Figure 7: Cell cycle analysis: Flow cytometry for cell cycle analysis of the (A) control
cells after 24 h and (B) cells + 20 µg/mL SP-Cou-Cbl, irradiated for 30 min and incubated
for 24 h.
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Conclusion:
Here, we have demonstrated for the first time a spiropyran
locked coumarin system for efficient photoregulated drug
delivery. It can locate tumor by the appearance of blue
fluorescent color due to the unlocking of the coumarin ring by
the acidic nature of tumor cell. Drug can be released in a dose
controlled manner by the use of externally regulated light
source. High fluorescent nature and promising biocompatibility
make SPꢀCouꢀCbl system suitable for cell imaging and MDAꢀ
MBꢀ231 cells was used for in vitro studies.
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Acknowledgements
We thank the DST (SERB) for financial support and the DSTꢀ
FIST for 400 MHz NMR. We are grateful to the Indian Institute
of Technology Kharagpur and UGC (India) for our research
fellowships.
22. S. Atta, M. Ikbal, A. Kumar, N.D. P. Singh, J. of
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Notes and references
[a] Prof. N. D. Pradeep Singh, Shrabani Barman, Sandipan Biswas,
Department of Chemistry, Indian Institute of Technology Kharagpur
721302,
West
Bengal,
India
[b] Prof.T. K. Maiti, Joyjyoti Das. Department of Biotechnology, Indian
Institute of Technology Kharagpur 721302, West Bengal, India
†
Electronic Supplementary Information (ESI) available: [General
experimental procedure, Characterization of compounds 2, 3, 4 and 5, the
physical characteristics of compound 5, HPLC chromatogram of photolysis
and ring opening of compound 5.
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