PURIFICATION AND PROPERTIES OF LIPOXYGENASE IN MARCHANTIA POLYMORPHA CULTURED CELLS

Article abstract of DOI:10.1016/0031-9422(91)84196-Y

Lipoxygenase activity has been detected in cultured cells of a bryophyte, Marchantia polymorpha (liverwort).The activity was enhanced in the stationary phase.Most of the activity occurred in the cytosolic fraction.The lipoxygenase was purified to homogeneity by ammonium sulphate fractionation and hydrophobic, ion-exchange, and gel filtration chromatography.From the SDS-polyacrylamide gel electrophoresis, the M_r of the lipoxygenase was estimated as 109600.The pH optimum was ca 9.0 and the pI was 4.8.This enzyme formed mainly 13-(S)-hydroperoxy-(9Z,11E)-octadecadienoic acid from linoleic acid.It showed twice the activity for γ-linolenic acid than for linoleic acid. Key Word Index - Marchantia polymorpha; Marchantiaceae; liverwort; purification; lipoxygenase.