Novel fungal metabolites as cell wall active antifungals: Fermentation, isolation, physico-chemical properties, structure and biological activity

Article abstract of DOI:10.7164/antibiotics.54.74

Two novel antifungal compounds, 1 (SCH 466457), and 2 (SCH 466456), active in a cell wall assay, were isolated from the fermentation broth of an unidentified fungus. The active compounds were separated from the broth filtrate by adsorption on a macroreticular resin and were purified on reverse phase HPLC. Detailed mass spectrometric and NMR experiments and degradative studies helped in elucidating the structures of these compounds. The compounds were identified to be peptides containing amino acids such as alanine, aminoisobutyric acid, proline, leucine, valine, glycine and a previously identified β-keto acid, 2-methyl 3-oxotetradecanoic acid.⁵) Both compounds were active against Candida, dermatophytes and Aspergillus (Geometric Mean MIC's, 8.9, 20 and 16 μg/ml, and 64, 128 and 23 μg/ml, respectively for 1 and 2).