acid (5% in distilled water, 0.1 cm3, 0.185 g) was added. After 20
min the precipitated protein was removed by centrifugation.
The solution was filtered and neutralised with NaOH (0.1 M).
References
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Ozonolysis of [3H]HMTDP 20
Ozonolysis of the [3H]HMTDP solution (3.7 cm3) to which had
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The p-bromophenacyl ester 21 of [2-3H]glycolic acid (14 mg,
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Acknowledgements
We thank the EPSRC for financial support and Professor
G. Hübner for a generous gift of pyruvate decarboxylase from
Saccharomyces cerevisiae.
2324
J. Chem. Soc., Perkin Trans. 1, 2000, 2317–2324