6
57
some 2H. Since Gln2 maps to barley chromosome 2HL Devos KM, Wang ZM, Beales J, Sasaki T, Gale MD (1998) Com-
parative genetic maps of foxtail millet (Setaria italica) and
(
GrainGenes 2000), it would appear that this primer set
rice (Oryza sativa). Theor Appl Genet 96:63–68
Erpelding JE, Blake NK, Blake TK, Talbert LE (1996) Transfer of
sequence tagged site PCR markers between wheat and barley.
Genome 39:802–810
directs amplification from the Gln2 locus in both barley
and L. perenne.
The current linkage maps available for Lolium are
based upon RFLP, RAPD, AFLP and a small number of
isozyme and EST markers (Hayward et al. 1998; Bert
et al. 1999). Few of the RFLP markers mapped in Lolium
have been mapped in other members of the Poaceae.
While RAPD and AFLP markers are highly polymorphic
and provide a ready source of polymorphic markers in
forage grasses (Charmet et al. 1997; Bert et al. 1999;
Kolliker et al. 1999), their transfer between populations is
likely to be problematic. Moreover, both RAPDs and
AFLPs are generally dominant, and are expected to pro-
vide little or no information regarding synteny. Conse-
quently, there is no possibility of aligning current Lolium
GrainGenes (2000) The Triticeae Genome Gopher. Accessible via
Internet Gopher, host graingenes.cit.cornell.edu, port 70,
menu: Grains files to browse
Hayward MD, McAdam NJ, Jones JG, Evans C, Evans GM,
Forster JW, Ustin A, Hossain KG, Quader B, Stammers M,
Will JK (1994) Genetic markers and the selection of quantita-
tive traits in forage grasses. Euphytica 77:269–275
Hayward MD, Forster JW, Jones JG, Dolstra O, Evans C,
McAdam NJ, Hossain KG, Stammers M, Will JK, Humphreys
MO, Evans GM (1998) Genetic analysis of Lolium. I. Identifi-
cation of linkage groups and the establishment of a genetic
map. Plant Breed 117:451–455
Islam AKMR (1983) Ditelosomic additions of barley chromo-
somes to wheat. Proc 6th Int Wheat Genet Symp, Kyoto,
Japan, pp 233–238
linkage maps with those of rice, barley or wheat, al- Islam AKMR, Shepherd KW, Sparrow DHB (1981) Isolation and
characterization of euplasmic wheat-barley chromosome addi-
though ongoing mapping of heterologous anchor RFLP
probes in L. perenne by a number of research groups will
ultimately allow the alignment of maps to those of other
Poaceae species. Data presented in this paper suggest
that the probability of amplifying homologous loci in L.
perenne using primers derived from wheat and barley
tion lines. Heredity 46:161–174
Kolliker R, Stadelmann FJ, Reidy B, Nosberger J (1999) Genetic
variability of forage grass cultivars: a comparison of Festuca
pratensis Huds., Lolium perenne L., and Dactylis glomerata L.
Euphytica 106:261–270
Kurata N, Moore G, Nagumara Y, Foote T, Yano M, Minobe Y,
Gale M (1994) Conservation of genome structure between rice
and wheat. Bio/Technology 12:276–278
(and perhaps other grass species) is approximately 50%.
Given that the ability to predict STS location in wheat Langridge P, Baumann U, Juttner J (1999) Revisiting and revising
based upon STS location in barley is 75% (Erpelding et
al. 1996), it would seem reasonable to assume that STS
location in barley will be an accurate predictor of STS lo-
cation in L. perenne. Significantly, this work indicates
that it should be possible to assemble a panel of “anchor”
STS loci for comparative mapping in forage grasses.
the self-incompatibility genetics of Phalaris coerulescens.
Plant Cell 10:1826–1827
Laurie DA (1997) Comparative genetics of flowering time. Plant
Mol Biol 35:167–177
Li X, Paech N, Nield J, Hayman D, Langridge P (1997) Self-in-
compatibility in the grasses: evolutionary relationships of the
S gene from Phalaris coerulescens to homologous sequences
in other grasses. Plant Mol Biol 34:223–232
Mano Y, Sayed-Tabatabaei BE, Graner A, Blake T, Takaiwa F,
Oka S, Komatsuda T (1999) Map construction of sequence-
tagged sites (STSs) in barley (Hordeum vulgare L.). Theor
Appl Genet 98:937–946
Marino CL, Nelson JC, Lu YH, Sorrells ME, Leroy P, Tuleen NA,
Lopes CR, Hart GE (1996) Molecular genetic maps of the
group-6 chromosomes of hexaploid wheat (Triticum aestivum
L. em. Thell.). Genome 39:359–366
Michalek W, Künzel G, Graner A (1999) Sequence analysis and
gene identification in a set of mapped RFLP markers in barley
(Hordeum vulgare). Genome 42:849–853
Nelson JC, Van Deynze AE, Autrique E, Sorrells ME, Lu YH,
Merlino M (1995a) Molecular mapping of wheat. Homoeolog-
ous group 2. Genome 38:516–524
Acknowledgements We thank Dr. Y. Mano for kindly providing
primer samples for barley STS markers and Dr. L. Talbert for pro-
viding primer samples for T. tauchii STS markers. This research
was supported by a grant provided by the Danish Seed Council
and the Danish Ministry of Food, Agriculture and Fisheries.
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