Chem. Biodiversity 2021, 18, e2100403
νmax 3400.6, 1649.6, 1026.0, 999.7, 827.2, 766.2 cmÀ 1;
UV λmax 201 (3.72) nm; HRMS: m/z 583.0975 [M+Na]+,
calc. for 583.0956. 1H- and 13C-NMR data: see Table 1.
References
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Cytotoxic Activity Assay
Lung carcinoma A549 cells were used in the cytotox-
icity assay. A549 cells were cultured in DEME medium
supplemented with 10% fetal bovine serum in 5%
°
CO2 at 37 C in a 96-well plate, each well was a plate
with 10×105 cells. After cell attachment overnight and
removed the medium, A549 cells were exposed to the
test compounds at concentrations of 3.125, 6.25, 12.5,
25, 50 μM in triplicate for 72 h, and the positive control
Taxol (antiproliferative activity against A549 cell lines
with an IC50 value of 0.015�0.005 μM). The cytotox-
icity assay was performed using the MTT (3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide). After the addition of a 20 μL MTT solution
(5 mg/mL), cells were incubated for 4 h. After adding
100 mL DMSO, cells were shaken for 15 min to mix
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Acknowledgments
The work was supported by the China Postdoctoral
Science Foundation (2020M673566XB), the Application
basic research project of the science and technology
department of Sichuan Province (2019YJ0333), National
Natural Science Foundation of China (81503200).
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He, D. L. Guo, S. J. Xiao, ‘Dendrobine-type alkaloids and
bibenzyl derivatives from Dendrobium findlayanum’, Fito-
terapia 2020, 142, 104497.
Author Contribution Statement
All the authors have contributed significantly to the
submitted work. J. Y., D.G., X.F., W.G., L.Z., and H.Z.
performed the experiments, analyzed the data, and
wrote the article. J. P. and Y.D. gave guidance and
suggestions for the experiment and the article. C.P.
and D.L. conceived and designed the experiments.
Received May 31, 2021
Accepted August 6, 2021
(5 of 5) e2100403
© 2021 Wiley-VHCA AG, Zurich, Switzerland