Improved synthesis of 1-O-acyl-β-D-glucopyranose tetraacetates

Article abstract of DOI:10.3390/molecules22040662

An improved synthesis of 1-O-acyl glucosyl esters that avoids the use of expensive Ag reagents as well as the hydrolysis of unstable glucosyl bromides is reported. Notably, β-configuration products were obtained exclusively in good yields.

Full text of DOI:10.3390/molecules22040662

molecules
Article
Improved Synthesis of
1-O-Acyl-β-D-Glucopyranose Tetraacetates
Yu Chen 1,2, Huan Lu 2, Yanyu Chen 1, Wansheng Yu 2, Hui Dai 2 and Xianhua Pan 1,2,
*
1
School of Perfume and Aroma Technology, Shanghai Institute of Technology, 100 Haiquan Rd.,
Shanghai 201418, China; chenyu@sit.edu.cn (Y.C.); 156071203@mail.sit.edu.cn (Y.C.)
Shanghai Research Institute of Fragrance and Flavor Industry, 480 Nanning Rd., Shanghai 200232, China;
aijiudu@sina.com (H.L.); yu102658@126.com (W.Y.); ddai_hui@126.com (H.D.)
Correspondence: panxh@sit.edu.cn; Tel./Fax: +86-21-5496-1786
2
*
Academic Editor: Roman Dembinski
Received: 16 February 2017; Accepted: 17 April 2017; Published: 21 April 2017
Abstract: An improved synthesis of 1-O-acyl glucosyl esters that avoids the use of expensive Ag
reagents as well as the hydrolysis of unstable glucosyl bromides is reported. Notably,
products were obtained exclusively in good yields.
β-configuration
Keywords: glucosyl esters; glucosyl bromide; aromatic acids; aliphatic acids
1. Introduction
Numerous glycosyl esters have been investigated because of their biologically activity.
Compounds such as tuliposide-A and tuliposide-B show bacteriotoxic and fungitoxic effects [ ].
Some saturated fatty acid glycosyl esters were examined for antitumor activity [ ]. In addition,
1,2
3
glycosyl esters have also been used in cosmetics, detergents, oral-care products and medical supplies
as flavor precursors.
The fact that few 1-O-acyl glycosyl esters have been found in Nature, has led to the development
of various synthetic methods to access these compounds. The Koenigs-Knorr reaction using glycosyl
bromide and an acid is the most attractive. Several publications have disclosed the glycosylation of
carboxylic acids promoted by Ag catalysts through Koenigs-Knorr reaction (1a) [4–6]. However, the
need for expensive Ag catalysts (at least one equivalent) has limited its application (Scheme 1).
Scheme 1. Glycosylation of carboxylic acids promoted by Ag catalysts.
Therefore, other alternative methods have been reported (Scheme 2), involving compounds
such as orthoesters [
trichloroacetimidates [16], etc. In addition, the activation of the carboxylic acid group using the
Mitsunobu protocol [17], DCC [18 20] or EDCI [21] were also explored. However several drawbacks
7,8], trifluoroacetates [9,10], TMSET glycosides [11], glucosyl fluorides [12–15],
–
including troublesome preparation of the intermediates, the use of toxic reagents or the harsh
conditions of these methods, make the reactions challenging.
Molecules 2017, 22, 662; doi:10.3390/molecules22040662
www.mdpi.com/journal/molecules

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Scheme 2. Other alternative methods.
2. Results and Discussion
Among the 1-O-acyl glycosyl esters, 1-O-acyl glucosyl esters are the most important and
common. The formation of 1-O-acyl glucosyl esters by condensation of acids with glucosyl bromide in
aqueous/DCM in the presence of an inorganic base seemed to be a good choice [22], but in our hands
this reaction gave low yields for most substrates when run on a larger scale (1 g), with lactol
mixture) being formed during the condensation. The reason was found to be the hydrolysis of the
glucosyl bromide in the presence of H2O. Herein, we describe the improvement of this synthesis and
4 (as an
α/β
1
preparation of a series of glucosyl esters.
We started to study this reaction with benzoic acid (2) which was reacted with α-glucosyl bromide
1
in the presence of tricaprylylmethylammonium chloride (a mixture of C8-C10 species in which C8
is dominant, sold under the brand name Aliquat 336®) as the phase transfer catalyst (PTC). From
Table 1, we can see that the reaction was greatly influenced by water. The more water added, the
more compound
the solvent, product
4
was formed in the reaction (Table 1, entries 1–3). When only DCM was used as
was obtained in high yield, with less than 5% of the lactol (Table 1, entry 4).
3
4
Considering 0.5 equiv. of water would be formed in the reaction with K2CO3 itself, 4 Å molecular
sieves (4 Å MS) were added, which increased the yield by 6% (Table 1, entry 5). It was found that
K2CO3 was the best base after comparing different ones according to the yield and the cost (Table 1,
entries 6–10). The reaction was completely suppressed when NaOH or Et3N were used as the base
with recycled compound
1, probably because of the instability of the PTC in the presence of stronger
base (Table 1, entry 9) or due to the weaker basicity of Et3N (Table 1, entry 10). Notably, compared and

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in contrast with the known data [
9
–
11,
23,
24] the
β-configuration product was exclusively obtained
through SN2 substitution,.
Table 1. The influence of water and the screening of base for the reaction of 1 with 2 a.
3 b
4 b
Entry
Base
H2O
1
2
3
4
5
6
7
8
9
K2CO3
K2CO3
K2CO3
K2CO3
K2CO3
Na2CO3
NaHCO3
Cs2CO3
NaOH
Et3N
5 mL (278 mmol) c
2.5 mL (139 mmol)
0.5 mL (27.8 mmol)
-
35%
54%
78%
88%
94%
80%
69%
90%
NR
55%
35%
12%
4%
trace
trace
trace
trace
trace
trace
d
-
-
-
-
d
d
d
d
-
d
10
NR
-
a
The reaction was conducted with
1
(2.5 mmol),
2
(5 mmol), base (5 mmol) and Aliquat 336® (0.25 mmol) in 35 mL
DCM with or without H2O. b Isolated yield. About 115 equiv. of H2O to glucosyl bromide was used according to
reference 22. 0.25 g 4 Å molecular sieve was added.
c
d
Next, the PTC and the solvent were varied. From Table 2, it seems that the reaction did not
happen without a PTC. Only 10% mol of a PTC such as tetrabutylammonium bromide (TBAB),
tetraethylammonium bromide (TEAB), benzyltriethylammonium chloride (BTEAC), hexadecyl-
trimethylammonium bromide (CTMAB) led the reaction to give the product in high yield (Table 2,
entries 1–4).
Table 2. Variation of PTC and the solvent a.
3 b
Entry
PTC
Solvent
1
2
3
4
5
6
7
8
TBAB
TEAB
BEAC
CMAB
-
TEAB
TEAB
TEAB
DCM
DCM
DCM
DCM
DCM
THF
99%
99%
96%
97%
NR
<10%
78%
<10%
CH3CN
DMF
a
The reaction was conducted with
1 (2.5 mmol), 2 (5 mmol), K2CO3 (5 mmol), PTC (0.25 mmol) and 0.25 g 4 Å MS
b
in 35 mL solvent. Isolated yield.
In the comparison of the solvents, DCM proved to be the best solvent (Table 2, entries 6–8).
The role of the PTC is unclear, but it seems to increase the solubility of carboxylate formed at the
beginning of the reaction, due to the quite low solubility of the latter.
Next, various acids were chosen to verify the scope of this reaction (Tables 3 and 4). Aromatic
acids with different kind of substituent groups at different positions on benzene ring, gave the desired

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product in 80–99% yield. For example, electron-donating groups, such as methoxy, benzyloxy or
methyl could all make the reaction happen smoothly (Table 3, entries 1–5). Electron-withdrawing
group also produced the corresponding products in 85–99% yield (Table 3, entries 6–8). Similarly,
β-naphtoic acid gave product 22 quantitatively (Table 3, entry 9). In the comparison experiments, the
yield decreased evidently because compound is sensitive to hydrolysis as described before when the
1
reaction was conducted in the presence of water (Table 3, entry 1, 3, 7 and 9). The β-configuration of
the products was confirmed by 2D-NMR data of compound 8 [25]
Table 3. The reaction of glucosyl bromide 1 with aromatic acids a.
Yield b (%)
Entry
Aromatic Acids
Product
1
95 c
5
6
2
3
98
7
9
8
91 c
10
4
80
96
11
12
5
6
13
15
14
16
99
7
8
85 c
17
18
20
90
19
21
9
99 c
22
a
The reaction was conducted with
1 (2.5 mmol), aromatic acid (5 mmol), K2CO3 (5 mmol), TEAB (0.25 mmol) and
0.25 g 4 Å MS in 35 mL DCM. b Isolated yield. 28–54% yields of these compounds were obtained when 5 mL H2O
c
was added in the reaction mixture.

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Table 4. The reaction of glucosyl bromide 1 with aliphatic acids a.
Aliphatic Acids Product
Yield b (%)
Entry
1
95 c
23
24
2
97
25
26
28
30
3
4
5
91
99 c
72
27
29
31
33
32
34
36
38
6
7
8
92 c
96
35
37
79
9
94
39
40
10
99 c
41
43
42
44
11
78
a
The reaction was conducted with
1
(2.5 mmol), aliphatic acid (5 mmol), K2CO3 (5 mmol), TEAB (0.25 mmol) and
0.25 g 4 Å MS in 35 mL DCM. b Isolated yield. 40–58% yields of these compounds were obtained when 5 mL H2O
c
was added to the reaction mixture.

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Not only aromatic acids, but aliphatic acids could be used in the reaction too. The results are listed
in Table 4. Phenylacetic acid (23) and 2,4,5-trifluorophenylacetic acid (25) provided the corresponding
product in no less than 95% yield (Table 4, entries 1–2). Good results were also obtained using other
aliphatic acids. For example, isobutyric acid (27) and isovaleric acid (29) gave the products in more
than 90% yield respectively. Lower yield was obtained for pivalic acid (31), probably due to the steric
hindrance (Table 4, entries 3–5).
In addition, a long chain glucosyl ester was prepared in good yield from acid 33 (Table 4, entry 6).
Satisfactorily, this reaction could be also be extended to aliphatic acids with olefins and rings (Table 4,
entries 7–11). For the same reason as before, the results were not good when water was added in the
comparison sample due to the hydrolysis of 1 (Table 4, entry 1, 4, 6 and 10).
It is noteworthy that when we tried to prepare two 1-O-acyl-β-D-glucopyranose tetraacetates on
a large scale ( and 24, more than 100 g), these could be purified without column chromatography.
3
It seems that this method could be applicable in industrial manufacture due to the high yields generally
obtained. The scaled-up synthesis of other compounds and the study of other kinds of glycosylation
are now underway.
3. Materials and Methods
3.1. General Methods
All solvents and reagents, except for compound 1, were purchased from the commercial supplier
Tansoole (Shanghai, China) and were used without further purification. Compound
1
was prepared
◦
according to the known method [26]. 4 Å MS were activated at 600 C for one-day and kept in a
dessicator. The progress of the reactions was assessed by thin-layer chromatography (TLC) with GF254
silica-gel precoated sheets using EtOAc/hexane as eluent. Column chromatography was performed
1
on silica gel (200–300 mesh) using EtOAc/hexane or EtOAc/petroleum ether as eluent. H (400 MHz)
and 13C (100 MHz) NMR spectra were recorded on an Avance 400 spectrometer (Bruker, Karlsruhe,
Germany) in CDCl3 using tetramethylsilane (TMS) as internal standards. 2D-NMR was recorded on a
Bruker Avance 500 spectrometer. J values were given in Hertz. Mass spectra a high resolution mass
spectra were recorded on an ESQUIRE-LC mass spectrometer (Agilent, Palo Alto, CA, USA). Elemental
analysis was performed on an Elemental Vario-III CHN analyzer (Elementar Analysensysteme
GmbH, Hanau, Germany). Optical rotations were measured on a WZZ-2S polarimeter (Suoguang
Electric Tech Co., Shanghai, China) in DCM, with concentrations denoted in g/100 mL. Melting
points were determined on a SGW-X4 melting point instruments (Shenguang Instrument Co., Ltd.,
Shanghai, China).
3.2. General Procedure for the Synthesis of 1-O-Acyl-β-D-Glucopyranose Tetraacetates
A mixture of glucosyl bromide 1 (1.03 g, 2.5 mmol), acid (5.0 mmol), K2CO3 (0.69 g, 5.0 mmol),
TEAB (0.05 g, 0.25 mmol) and 4 Å MS (0.25 g) in 35 mL DCM was stirred 24–48 h at room temperature.
Next, the insoluble substances, made up of the slightly soluble potassium carboxylate, 4 Å MS and
other salts, were filtered off. The filtrate was washed with water, and the separated organic layer was
then washed with 25% aqueous K2CO3 to removed any remaining potassium carboxylate. After drying
over MgSO4 and concentration in vacuo, the residue was purified via silica gel column chromatography
using EtOAc/hexane or EtOAc/petroleum ether (1:10 to 1:1) as eluents to yield the desired product.
3.3. Scaled-Up Synthesis of Compound 3
A mixture of glucosyl bromide
1 (150.0 g, 0.36 mol), benzoic acid 2 (89.0 g, 0.73 mol), K2CO3
(100.7 g, 0.73 mol), TEAB (7.5 g, 36 mmol) and 4 Å MS (36.0 g) in 5 L DCM was stirred 24 h at room
temperature. Next, the insoluble substances, made up of the slightly soluble potassium benzoate, 4 Å
MS and other salts, were filtered off. The filtrate was washed by water, and the separated organic
layer was then washed with 25% aqueous K2CO3 to remove any remaining potassium benzoate. After

Molecules 2017, 22, 662
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drying over MgSO4 and concentration in vacuo, the crude was purified in refluxing EtOH to give
a white solid in 89% yield after cooling down.
3 as
3.4. Scaled-Up Synthesis of Compound 24
A mixture of glucosyl bromide 1 (150.0 g, 0.36 mol), phenylacetic acid 23 (99.3 g, 0.73 mol), K2CO3
(100.7 g, 0.73 mol), TEAB (7.5 g, 36 mmol) and 4 Å MS (36.0 g) in 5 L DCM was stirred 26 h at room
temperature. After work-up as described in Section 3.3, compound 24 was obtained as a white solid in
78% yield after cooling down.
1-O-Benzoyl-2,3,4,6-tetra-O-acetyl-
c = 0.5, DCM); 1H-NMR:
= 1.99 (s, 3H), 2.04 (s, 3H), 2.05 (s, 3H), 2.07 (s, 3H), 3.93–3.97 (m, 1H), 4.14
(dd, J = 2.0, 12.8 Hz, 1H), 4.33 (dd, J = 4.4, 12.4 Hz, 1H), 5.18–5.23 (m, 1H), 5.34–5.37 (m, 2H), 5.93–5.95
(m, 1H) [ 11
27], 7.46 (t, J = 8.0 Hz, 1H), 7.61 (t, J = 7.2 Hz, 1H), 8.05 (dd, J = 1.2, 8.0 Hz, 2H); 13C-NMR:
β-D-glucopyranose (3
). White solid, m.p. 143–144 ◦C; [α]D20 = +55.6
(
δ
9,
,
δ
= 20.45, 20.49, 20.51, 20.58, 61.4, 67.9, 70.1, 72.6, 72.7, 92.2 [23,28], 128.4, 128.6, 130.1, 133.9, 164.4, 169.3,
169.4, 170.0, 170.5; ESI-MS (m/z) 475 [M + Na]+; HRMS calcd. for C21H24O11 452.1330, found 452.1321;
Anal. Calcd. for C21H24O11 (%): C, 55.75; H, 5.35. Found: C, 55.85; H, 5.26.
1-O-(2-Methoxybenzoyl)-2,3,4,6-tetra-O-acetyl-
= +71.2 (c = 0.5, DCM); 1H-NMR:
= 2.01 (s, 3H), 2.04 (s, 3H), 2.05 (s, 3H), 2.07 (s, 3H), 3.91 (s, 3H),
β-D-glucopyranose (6
). White solid, m.p. 89–90 ◦C; [α]D20
δ
3.91–3.94 (m, 1H), 4.14 (dd, J = 2.0, 12.8 Hz, 1H), 4.33 (dd, J = 4.4, 12.4 Hz, 1H), 5.17–5.22 (m, 1H),
5.31–5.33 (m, 2H), 5.95–5.97 (m, 1H), 6.97–7.01 (m, 2H), 7.50–7.55 (m, 1H), 7.87 (dd, J = 1.6, 8.0 Hz, 2H);
13C-NMR:
δ = 20.44, 20.46, 20.5, 20.6, 55.7, 61.5, 67.8, 70.2, 72.6, 72.8, 91.8, 112.0, 117.4, 120.1, 132.4,
134.8, 160.1, 163.2, 169.2, 169.3, 170.0, 170.4; ESI-MS (m/z) 505 [M + Na]+; Anal. Calcd. for C22H26O12
(%): C, 54.77; H, 5.43. Found: C, 54.90; H, 5.30.
1-O-(3,4-Dimethoxybenzoyl)-2,3,4,6-tetra-O-acetyl-
α]2D0 = +75.6 (c = 0.5, DCM); 1H-NMR:
= 1.99 (s, 3H), 2.04 (s, 3H), 2.06 (s, 3H), 2.08 (s, 3H), 3.94 (s, 3H),
3.95 (s, 3H), 3.96–3.98 (m, 1H, H-5), 4.14 (dd, J = 2.0, 12.4 Hz, 1H, H-6), 4.33 (dd, J = 4.4, 12.8 Hz, 1H,
H-6), 5.18–5.23 (m, 1H, H-4), 5.34–5.36 (m, 2H, H-3 and H-2), 5.88–5.90 (m, 1H, H-1), 6.91 (d, J = 8.4 Hz
1H), 7.54 (d, J = 2.0 Hz, 1H), 7.70 (dd, J = 1.6, 8.0 Hz, 2H); 13C-NMR:
= 20.5, 20.6, 55.9, 56.0, 61.4 (C-6),
β-D-glucopyranose (8
). White solid, m.p. 135–136 ◦C;
[
δ
,
δ
67.9 (C-4), 70.1 (C-2), 72.5 (C-3), 72.6 (C-5), 92.2 (C-1), 110.4, 112.2, 120.6, 124.5, 148.7, 153.8, 164.1, 169.3,
169.4, 170.0, 170.5; ESI-MS (m/z) 535 [M + Na]+; Anal. Calcd. for C23H28O13 (%): C, 53.91; H, 5.51.
Found: C, 54.00; H, 5.65.
1-O-(4-Benzyloxy-3-methoxybenzoyl)-2,3,4,6-tetra-O-acetyl-
β
-D-glucopyranose (10). White solid, m.p.
◦
126–127 C; [α]2D0 = +27.5 (c = 0.5, DCM); 1H-NMR:
δ = 1.98 (s, 3H), 2.04 (s, 3H), 2.05 (s, 3H), 2.07 (s, 3H),
3.92–3.96 (m, 1H), 3.94 (s, 3H), 4.14 (dd, J = 2.4, 12.4 Hz, 1H), 4.33 (dd, J = 4.4, 12.4 Hz, 1H), 5.17–5.21
(m, 1H), 5.2 (s, 3H), 5.33–5.35 (m, 2H), 5.87–5.89 (m, 1H), 6.91 (d, J = 8.8 Hz, 1H), 7.32–7.44 (m, 5H),
7.55 (d, J = 1.6 Hz, 1H), 7.62 (dd, J = 1.6, 8.4 Hz, 1H); 13C-NMR:
δ = 20.6, 20.7, 56.1, 61.5, 67.9, 70.2, 70.7,
72.5, 72.7, 92.2, 112.5, 112.7, 120.9, 124.3, 127.2, 128.1, 128.7, 136.1, 149.2, 152.9, 164.2, 169.3, 169.4, 170.0,
170.6; ESI-MS (m/z) 611 [M + Na]+; Anal. Calcd. for C29H32O13 (%): C, 59.18; H, 5.48. Found: C, 59.01;
H, 5.60.
1-O-(3,4,5-Trimethoxybenzoyl)-2,3,4,6-tetra-O-acetyl-
α]2D0 = +26.9 (c = 0.5, DCM); 1H-NMR:
= 1.97 (s, 3H), 2.02 (s, 3H), 2.03 (s, 3H), 2.05 (s, 3H), 3.85–3.95
(m, 1H), 3.88 (s, 9H), 4.12 (d, J = 12.8 Hz, 1H), 4.32 (dd, J = 4.4, 12.8 Hz, 1H), 5.16–5.20 (m, 1H), 5.30–5.36
β
-D-glucopyranose (12). White solid, m.p. 55–56 ◦C;
[
δ
(m, 2H), 5.83–5.85 (m, 1H), 7.28 (s, 2H); 13C-NMR:
δ = 20.4, 20.5, 20.6, 56.2, 60.8, 61.4, 67.9, 70.2, 72.3,
72.6, 92.4, 107.3, 123.1, 142.9, 152.9, 164.0, 169.3, 169.4, 170.0, 170.5; ESI-MS (m/z) 565 [M + Na]+; Anal.
Calcd. for C24H30O14 (%): C, 53.14; H, 5.57. Found: C, 53.31; H, 5.71.
1-O-(2,5-Dimethylbenzoyl)-2,3,4,6-tetra-O-acetyl-
DCM); 1H-NMR:
= 1.94 (s, 3H), 1.97 (s, 3H), 1.98 (s, 3H), 2.00 (s, 3H), 2.28 (s, 3H), 2.48 (s, 3H), 3.84–3.88
(m, 1H), 4.07 (dd, J = 2.4, 12.4 Hz, 1H), 4.26 (dd, J = 4.4, 12.4 Hz, 1H), 5.11–5.16 (m, 1H), 5.25–5.28 (m,
β
-D-glucopyranose (14). Syrup; [α]2D0 = +78.3 (c = 0.5,
δ

Molecules 2017, 22, 662
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2H), 5.86–5.88 (m, 1H), 7.07 (d, J = 8.0 Hz, 1H), 7.18 (d, J = 8.0 Hz, 1H), 7.69 (s, 1H); 13C-NMR:
δ
= 20.53,
20.56, 20.6, 20.7, 21.4, 21.5, 61.5, 67.9, 70.3, 72.7, 72.9, 91.9, 127.0, 131.7, 131.8, 133.9, 135.6, 138.4, 165.0,
169.3, 169.5, 170.2, 170.7; ESI-MS (m/z) 503 [M + Na]+; Anal. Calcd. for C23H28O11 (%): C, 57.50; H,
5.87. Found: C, 57.60; H, 5.79.
1-O-(3-Bromobenzoyl)-2,3,4,6-tetra-O-acetyl-
= +50.7 (c = 0.5, DCM); 1H-NMR:
= 2.00 (s, 3H), 2.05 (s, 3H), 2.06 (s, 3H), 2.08 (s, 3H), 3.92–3.97 (m,
β
-D-glucopyranose (16). White solid, m.p. 119–120 ◦C; [α]D20
δ
1H), 4.14 (dd, J = 2.0, 12.8 Hz, 1H), 4.33 (dd, J = 4.4, 12.4 Hz, 1H), 5.17–5.22 (m, 1H), 5.33–5.35 (m, 2H),
5.92–5.94 (m, 1H), 7.35 (t, J = 8.0 Hz, 1H), 7.72–7.75 (m, 1H), 7.95–7.98 (m, 1H), 8.18 (t, J = 1.6 Hz, 1H);
13C-NMR:
δ = 20.50, 20.53, 20.55, 20.6, 61.4, 67.8, 70.1, 72.5, 72.7, 92.5, 122.6, 128.6, 130.2, 130.4, 133.0,
136.9, 163.2, 169.3, 169.4, 170.0, 170.5; ESI-MS (m/z) 553 [M + Na]+; Anal. Calcd. for C21H23BrO11 (%):
C, 47.47; H, 4.36. Found: C, 47.50; H, 4.41.
◦
1-O-(2-Chloro-4-fluorobenzoyl)-2,3,4,6-tetra-O-acetyl-
α]2D0 = +72.2 (c = 0.5, DCM); 1H-NMR:
β
-D-glucopyranose (18). White solid, m.p. 116–117 C;
[
δ
= 2.00 (s, 3H), 2.03 (s, 3H), 2.04 (s, 3H), 2.07 (s, 3H), 3.90–3.94
(m, 1H), 4.13 (dd, J = 2.0, 12.0 Hz, 1H), 4.32 (dd, J = 4.8, 12.4 Hz, 1H), 5.15–5.20 (m, 1H), 5.30–5.33 (m,
2H), 5.92–5.93 (m, 1H), 7.03–7.08 (m, 1H), 7.21 (dd, J = 6.8, 8.4 Hz, 1H), 7.97 (dd, J = 2.0, 6.0 Hz, 1H);
13C-NMR:
δ = 20.5, 20.6, 61.4, 67.7, 70.1, 72.6, 72.8, 92.3, 114.4 (d, J = 21.7 Hz), 119.0 (d, J = 24.5 Hz),
123.6 (d, J = 3.4 Hz), 134.5 (d, J = 9.9 Hz), 137.1 (d, J = 10.7 Hz), 161.8, 164.7 (d, J = 257.1 Hz), 169.2, 169.3,
170.0, 170.5; ESI-MS (m/z) 527 [M + Na]+; HRMS calcd for C21H22ClFO11 504.0808, found 504.0805;
Anal. Calcd. for C21H22ClFO11 (%): C, 49.96; H, 4.39. Found: C, 49.62; H, 4.46.
1-O-(3-Nitrobenzoyl)-2,3,4,6-tetra-O-acetyl-
= +32.9 (c = 0.5, DCM); 1H-NMR:
= 1.99 (s, 3H), 2.03 (s, 3H), 2.04 (s, 3H), 2.07 (s, 3H), 3.93–3.97 (m,
β
-D-glucopyranose (20). White solid, m.p. 109–110 ◦C; [α]D20
δ
1H), 4.13 (dd, J = 2.0, 12.0 Hz, 1H), 4.31 (dd, J = 4.4, 12.8 Hz, 1H), 5.17–5.21 (m, 1H), 5.33–5.35 (m,
2H), 5.94–5.96 (m, 1H), 7.68 (t, J = 8.0 Hz, 1H), 8.32–8.35 (m, 1H), 8.44–8.46 (m, 1H), 8.86–8.87 (m, 1H);
13C-NMR:
δ = 20.4, 20.5, 20.6, 61.4, 67.8, 70.1, 72.3, 72.8, 92.8, 125.1, 128.2, 130.0, 130.2, 135.5, 148.3,
162.5, 169.2, 169.4, 170.0, 170.5; ESI-MS (m/z) 520 [M + Na]+; Anal. Calcd. for C21H23NO13 (%): C,
50.71; H, 4.66; N, 2.82. Found: C, 50.65; H, 4.78; N, 2.70.
1-O-(2-Naphthoyl)-2,3,4,6-tetra-O-acetyl-
+47.8 (c = 0.5, DCM); 1H-NMR:
= 1.99 (s, 3H), 2.01 (s, 3H), 2.07 (s, 3H), 2.08 (s, 3H), 3.97–4.00 (m, 1H),
β =
-D-glucopyranose (22). White solid, m.p. 135–136 ◦C; [α]D20
δ
4.16 (dd, J = 2.0, 12.8 Hz, 1H), 4.35 (dd, J = 4.4, 12.4 Hz, 1H), 5.24 (t, J = 9.6 Hz, 1H), 5.35–5.44 (m, 2H),
6.01 (d, J = 8.0 Hz, 1H), 7.55–7.59 (m, 1H), 7.60–7.64 (m, 1H), 7.88–7.91 (m, 2H), 7.98 (d, J = 7.2 Hz, 1H),
8.04 (dd, J = 2.0, 8.8 Hz, 1H), 8.63 (d, J = 0.8 Hz, 1H); 13C-NMR:
δ = 20.51, 20.56, 20.58, 20.6, 61.5, 67.9,
70.3, 72.7, 72.8, 92.4, 125.1, 125.6, 126.9, 127.8, 128.5, 128.8, 129.6, 132.2, 132.3, 135.9, 164.7, 169.4, 169.5,
170.1, 170.6; ESI-MS (m/z) 525 [M + Na]+; Anal. Calcd. for C25H26O11 (%): C, 59.76; H, 5.22. Found: C,
59.89; H, 5.15.
1-O-(2-Phenylacetyl)-2,3,4,6-tetra-O-acetyl-
= +91.7 (c = 0.5, DCM); 1H-NMR:
= 1.76 (s, 3H), 1.99 (s, 3H), 2.03 (s, 3H), 2.09 (s, 3H), 3.66 (s, 2H),
3.82–3.86 (m, 1H), 4.12 (dd, J = 2.0, 12.8 Hz, 1H), 4.30 (dd, J = 4.4, 12.4 Hz, 1H), 5.10–5.15 (m, 2H), 5.21
(t, J = 8.8 Hz, 1H), 5.69 (d, J = 7.6 Hz, 1H), 7.25–7.34 (m, 5H); 13C-NMR:
= 20.2, 20.5, 20.6, 41.1, 61.4,
β
-D-glucopyranose (24). White solid, m.p. 108–109 ◦C; [α]D20
δ
δ
67.7, 69.9, 72.6, 72.7, 91.8, 127.4, 128.7, 129.2, 132.9, 169.0, 169.3, 169.4, 170.0, 170.5; ESI-MS (m/z) 489
[M + Na]+; HRMS calcd for C22H26O11 466.1481, found 466.1477; Anal. Calcd. for C22H26O11 (%): C,
56.65; H, 5.62. Found: C, 56.78; H, 5.50; Anal. Calcd. for C22H26O11 (%): C, 56.65; H, 5.62. Found: C,
56.59; H, 5.68.
1-O-(2-(2,4,5-Trifluorophenyl)acetyl)-2,3,4,6-tetra-O-acetyl-
β
-D-glucopyranose (26). White solid, m.p.
◦
100–101 C; [α]2D0 = +49.4 (c = 0.5, DCM); 1H-NMR:
δ = 1.99 (s, 3H), 2.01 (s, 3H), 2.03 (s, 3H), 2.09 (s, 3H),
3.67 (s, 2H), 3.82–3.87 (m, 1H), 4.12 (dd, J = 2.0, 12.8 Hz, 1H), 4.30 (dd, J = 4.4, 12.4 Hz, 1H), 5.10–5.15
(m, 2H), 5.25 (t, J = 8.8 Hz, 1H), 5.73 (d, J = 8.8 Hz, 1H), 6.91–6.98 (m, 1H), 7.07–7.13 (m, 1H); 13C-NMR:
δ
= 20.2, 20.4, 20.6, 33.5 (d, J = 1.9 Hz), 61.3, 67.6, 70.0, 72.5, 72.7, 92.2, 105.5 (dd, J = 20.5, 27.5 Hz), 116.5
(d, J = 17.5 Hz), 119.0 (dd, J = 5.6, 19.0 Hz), 146.6 (dd, J = 12.7, 243.1 Hz), 149.5 (d, J = 251.5 Hz), 156.0

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(dd, J = 10.4, 243.5 Hz), 167.9, 169.0, 169.3, 170.0, 170.5; ESI-MS (m/z) 543 [M + Na]+; Anal. Calcd. for
C22H23F3O11 (%): C, 50.77; H, 4.45. Found: C, 50.66; H, 4.50.
1-O-Isobutyryl-2,3,4,6-tetra-O-acetyl-
(c = 0.5, DCM); 1H-NMR:
= 1.16 (d, J = 7.2 Hz, 3H), 1.17 (d, J = 7.2 Hz, 3H), 2.02 (s, 6H), 2.04 (s, 3H),
2.09 (s, 3H), 2.57–2.64 (m, 1H), 3.83–3.87 (m, 1H), 4.12 (dd, J = 2.0, 12.4 Hz, 1H), 4.30 (dd, J = 4.4, 12.8
β
-D-glucopyranose (28). White solid, m.p. 108–109 ◦C; [α]D20 = +49.2
δ
Hz, 1H), 5.12–5.19 (m, 2H), 5.26 (t, J = 8.8 Hz, 1H), 5.72 (d, J = 8.4 Hz, 1H); 13C-NMR:
δ = 18.1, 18.7, 20.3,
20.4, 20.6, 33.7, 61.4, 67.8, 70.1, 72.6, 91.5, 169.0, 169.3, 169.9, 170.4, 174.9; ESI-MS (m/z) 441 [M + Na]+;
Anal. Calcd. for C18H26O11 (%): C, 51.67; H, 6.26. Found: C, 51.79; H, 6.20.
◦
β =
-D-glucopyranose (30). White solid, m.p. 73–74 C; [α]D20
1-O-(3-Methylbutanoyl)-2,3,4,6-tetra-O-acetyl-
+126 (c = 0.5, DCM); 1H-NMR:
= 0.95 (d, J = 6.8 Hz, 6H), 2.02 (s, 6H), 2.04 (s, 3H), 2.08 (s, 3H), 2.10–2.12
(m, 1H), 2.25 (d, J = 6.4 Hz, 2H), 3.83–3.87 (m, 1H), 4.11 (dd, J = 2.0, 12.4 Hz, 1H), 4.30 (dd, J = 4.4, 12.8
Hz, 1H), 5.11–5.17 (m, 2H), 5.26 (t, J = 9.2 Hz, 1H), 5.74 (d, J = 8.4 Hz, 1H); 13C-NMR:
= 20.3, 20.4,
δ
δ
20.5, 22.0, 25.4, 42.9, 61.4, 67.7, 70.1, 72.5, 72.7, 91.3, 168.9, 169.2, 169.9, 170.3, 170.8; ESI-MS (m/z) 455
[M + Na]+; Anal. Calcd. for C19H28O11 (%): C, 52.77; H, 6.53. Found: C, 52.90; H, 6.44.
1-O-Pivaloyl-2,3,4,6-tetra-O-acetyl-
(c = 0.5, DCM); 1H-NMR:
= 1.19 (s, 9H), 2.00 (s, 6H), 2.02 (s, 3H), 2.08 (s, 3H), 3.81–3.85 (m, 1H), 4.10
(dd, J = 2.4, 12.8 Hz, 1H), 4.29 (dd, J = 4.4, 12.0 Hz, 1H), 5.11–5.19 (m, 2H), 5.25 (t, J = 9.2 Hz, 1H), 5.66
β
-D-glucopyranose (32). White solid, m.p. 131–132 ◦C; [α]D20 = +187
δ
(d, J = 8.8 Hz, 1H); 13C-NMR:
δ = 20.3, 20.47, 20.48, 20.6, 26.6, 38.7, 61.4, 67.9, 70.0, 72.5, 72.6, 91.7, 169.0,
169.3, 170.0, 170.5, 176.4; ESI-MS (m/z) 455 [M + Na]+; Anal. Calcd. for C19H28O11 (%): C, 52.77; H,
6.53. Found: C, 52.89; H, 6.45.
1-O-Dodecanoyl-2,3,4,6-tetra-O-acetyl-
(c = 0.5, DCM); 1H-NMR:
= 0.88 (t, J = 9.2 Hz, 3H), 1.25–1.30 (m, 16H), 1.57–1.62 (m, 2H), 2.01 (s, 3H),
β
-D-glucopyranose (34). White solid, m.p. 53–54 ◦C; [α]D20 = +102
δ
2.02 (s, 3H), 2.03 (s, 3H), 2.09 (s, 3H), 2.34–2.38 (m, 2H), 3.82–3.87 (m, 1H), 4.11 (dd, J = 2.0, 12.0 Hz,
1H), 4.30 (dd, J = 4.4, 12.0 Hz, 1H), 5.11–5.16 (m, 2H), 5.26 (t, J = 9.2 Hz, 1H), 5.73 (d, J = 8.4 Hz, 1H);
13C-NMR:
δ = 14.0, 20.42, 20.46, 20.58, 20.59, 22.6, 24.5, 28.8, 29.1, 29.2, 29.3, 29.5, 31.8, 33.9, 61.4, 67.8,
70.2, 72.6, 72.7, 91.5, 169.1, 169.4, 170.0, 170.5, 171.7; ESI-MS (m/z) 553 [M + Na]+; Anal. Calcd. for
C26H42O11 (%): C, 58.85; H, 7.98. Found: C, 58.98; H, 7.88.
1-O-((E)-2-Methylpent-2-enoyl)-2,3,4,6-tetra-O-acetyl-
DCM); 1H-NMR:
= 1.05 (t, J = 8.0 Hz, 3H), 1.82 (s, 3H), 2.01 (s, 3H), 2.03 (s, 3H), 2.04 (s, 3H), 2.09
β
-D-glucopyranose (36). Syrup; [α]2D0 = +39.5 (c = 0.5,
δ
(s, 3H), 2.17–2.24 (m, 2H), 3.86–3.91 (m, 1H), 4.12 (dd, J = 2.0, 12.4 Hz, 1H), 4.31 (dd, J = 4.4, 12.4 Hz,
1H), 5.15 (t, J = 9.2 Hz, 1H), 5.21–5.32 (m, 2H), 5.75 (d, J = 8.0 Hz, 1H), 6.85 (dt, J = 1.2, 7.6 Hz, 1H);
13C-NMR:
δ = 11.9, 12.7, 20.43, 20.47, 20.48, 20.6, 22.1, 61.4, 67.9, 70.1, 72.5, 72.6, 91.9, 125.7, 147.2, 165.7,
169.1, 169.3, 170.0, 170.5; ESI-MS (m/z) 467 [M + Na]+, HRMS calcd for C20H28O11 444.1614, found
444.1618; Anal. Calcd. for C20H28O11 (%): C, 54.05; H, 6.35. Found: C, 53.95; H, 6.40.
1-O-((E)-Oct-2-enoyl)-2,3,4,6-tetra-O-acetyl-
1H-NMR:
= 0.86 (t, J = 7.2 Hz, 3H), 1.22–1.28 (m, 4H), 1.39–1.47 (m, 2H), 1.98 (s, 3H), 1.99 (s, 3H),
β
-D-glucopyranose (38). Syrup; [α]2D0 = +124 (c = 0.5, DCM);
δ
2.00 (s, 3H), 2.05 (s, 3H), 2.16–2.21 (m, 2H), 3.83–3.87 (m, 1H), 4.08 (dd, J = 2.0, 12.4 Hz, 1H), 4.27 (dd,
J = 4.4, 12.4 Hz, 1H), 5.10–5.18 (m, 2H), 5.25 (t, J = 9.2 Hz, 1H), 5.75 (d, J = 7.6 Hz, 1H), 5.76–5.80 (m,
1H), 7.01–7.09 (m, 1H); 13C-NMR:
δ = 13.8, 20.44, 20.48, 20.5, 20.6, 22.3, 27.4, 31.2, 32.3, 61.4, 67.8, 70.2,
72.6, 72.7, 91.6, 119.5, 153.1, 164.2, 169.2, 169.4, 170.0, 170.6; ESI-MS (m/z) 495 [M + Na]+; Anal. Calcd.
for C22H32O11 (%): C, 55.92; H, 6.83. Found: C, 55.85; H, 6.90.
1-O-((2E,6Z)-Nona-2,6-dienoyl)-2,3,4,6-tetra-O-acetyl-
DCM); 1H-NMR:
= 0.96 (t, J = 8.0 Hz, 3H), 2.01 (s, 3H), 2.02 (s, 3H), 2.02–2.05 (m, 2H), 2.04 (s, 3H),
β
-D-glucopyranose (40). Syrup; [α]2D0 = +86.3 (c = 0.5,
δ
2.08 (s, 3H), 2.19–2.23 (m, 2H), 2.25–2.29 (m, 2H), 3.85–3.89 (m, 1H), 4.11 (dd, J = 2.0, 12.4 Hz, 1H), 4.30
(dd, J = 4.4, 12.4 Hz, 1H), 5.12–5.21 (m, 2H), 5.27 (t, J = 9.2 Hz, 1H), 5.27–5.32 (m, 1H), 5.78 (d, J = 8.0 Hz,
1H), 5.80–5.86 (m, 1H), 7.04–7.11 (m, 1H); 13C-NMR:
δ = 14.1, 20.4, 20.5, 20.6, 25.3, 32.4, 61.4, 67.8,70.2,

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72.5, 72.7, 91.6, 120.0, 126.8, 133.0, 152.1, 164.0, 169.2, 169.3, 170.0, 170.5; ESI-MS (m/z) 507 [M + Na]+;
Anal. Calcd. for C23H32O11 (%): C, 57.02; H, 6.66. Found: C, 57.12; H, 6.63.
1-O-(Cyclopropanecarbonyl)-2,3,4,6-tetra-O-acetyl-
α]2D0 = +170.8 (c = 0.5, DCM); 1H-NMR:
= 0.94–0.97 (m, 2H), 1.03–1.10 (m, 2H), 1.63–1.67 (m, 1H),
β
-D-glucopyranose (42). White solid, m.p. 121–122 ◦C;
[
δ
2.02 (s, 3H), 2.03 (s, 3H), 2.04 (s, 3H), 2.09 (s, 3H), 3.82–3.86 (m, 1H), 4.11 (dd, J = 2.0, 12.0 Hz, 1H), 4.30
(dd, J = 4.4, 12.4 Hz, 1H), 5.11–5.17 (m, 2H), 5.26 (t, J = 9.6 Hz, 1H), 5.72 (d, J = 8.0 Hz, 1H); 13C-NMR:
δ
= 9.3, 12.7, 20.4, 20.6, 61.4, 67.7, 70.2, 72.5, 72.6, 91.5, 169.1, 169.3, 169.9, 170.4, 172.8; ESI-MS (m/z) 439
[M + Na]+; Anal. Calcd. for C18H24O11 (%): C, 51.92; H, 5.81. Found: C, 51.99; H, 5.75.
1-O-(Cyclohexanecarbonyl)-2,3,4,6-tetra-O-acetyl-
β
-D-glucopyranose (44). White solid, m.p. 111–112 ◦C;
α]2D0 = +96 (c = 0.5, DCM); H-NMR:
δ = 1.20–1.49 (m, 6H), 1.62–1.65 (m, 1H), 1.68–1.77 (m, 1H),
1
[
1.85–1.90 (m, 2H), 2.02 (s, 6H), 2.04 (s, 3H), 2.09 (s, 3H), 2.33–2.39 (m, 1H), 3.83–3.87 (m, 1H), 4.11 (dd,
J = 2.0, 12.0 Hz, 1H), 4.30 (dd, J = 4.4, 12.4 Hz, 1H), 5.11–5.18 (m, 2H), 5.26 (t, J = 9.6 Hz, 1H), 5.72 (d,
J = 8.4 Hz, 1H); 13C-NMR:
δ = 20.3, 20.4, 20.6, 24.9, 25.3, 25.5, 28.1, 28.7, 42.5, 61.4, 67.8, 70.1, 72.5, 72.6,
91.4, 169.1, 169.3, 170.0, 170.5, 173.8; ESI-MS (m/z) 481 [M + Na]+; Anal. Calcd. for C21H30O11 (%): C,
55.02; H, 6.60. Found: C, 55.16; H, 6.52.
4. Conclusions
The formation of 1-O-acyl glucosyl esters by condensation of acids with glucosyl bromide was
developed on a large scale in DCM without water. A diverse array of 1-O-acyl glucosyl esters were
prepared in good yields, which seems to indicate that our reaction conditions could be applied to a
broad substrate scope. In addition, scaled-up preparations were also successfully attempted.
Supplementary Materials: Supplementary materials can be accessed online.
Acknowledgments: We thank for the cooperation from the colleagues of the Analytical Department.
Author Contributions: Yu Chen and Xianhua Pan designed the experiments and wrote the paper. The
experimental work was conducted by Huan Lu, Yanyu Chen and Wansheng Yu under the supervision of Xianhua
Pan who is the corresponding author. Hui Dai contributed part of the data analysis.
Conflicts of Interest: The authors declare no conflict of interest.
References and Notes
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27. Anomeric H shift for
β-configuration is located at δ = 5.93 and anomeric H shift for α-configuration is located
at δ = 6.57 according to known data.
28. Anomeric C shift for β-configuration is located at δ = 92.3 according to known data.
Sample Availability: Samples of the compounds are available from the authors.
©
2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).