A convenient method for the preparation of oligonucleotide 5′-phosphates

Article abstract of DOI:10.1016/S0040-4020(96)00040-3

Oligodeoxyribonucleotides (4-, 5-, and 11-mer) bearing a 5′-phosphate group have been synthesized using 2-n-decylthioethyl as protecting group of the 5′-terminaI phosphate function. The lipophilic protecting group, which is easily available from 1-bromo-n-decane and 2-mercaptoethanol, allows a simple and efficient purification of the oligonucleotides. It is quantitatively removed by oxidation of the sulfide to the sulfoxide, followed by β-elimination in aqueous base media.