785 nm NIR laser at the power density of 0.5 W cm−2 for 5 min. The “Shu Guang” project (12SG07) supported by Shanghai Municipal
temperature of tumor site was recorded by an infrared camera. The
size of tumor and mice weight were measured every other day.
The tumor volume was calculated as V = d2 × D/2 (d: the shortest
diameter of tumor, D: the longest diameter of tumor). At 14th d, the
tumors and major organs (heart, liver, spleen, lung, and kidney) of
mice were dissected and all the mice were euthanized. All tumors
were weighed and parts of the organs and tumors were fixed in 4%
formaldehyde solution for tissue slices.
In Vivo Fluorescence Imaging of MMCNs: To investigate the
enrichment effect of ICG-loaded MMCNs in vivo, when tumor
size reached around 500 mm3, 100 μL (0.5 mg mL−1) ICG-loaded
MMCNs solution was injected into mice by tail intravenous. At
proper time intervals, the fluorescence images were recorded by
an optical and X-ray small animal imaging system (In Vivo Xtreme,
Bruker).
Biodistribution of Nanoparticles in Mice: Four weeks old male
mice were injected with ICG-loaded MMCNs intravenously. After
6 h, mice were treated with 785 nm laser at 0.5 W cm−2 power
density. Then at different time intervals (10 min, 48 h, 14 d), mice
were euthanized and major organs (heart, liver, spleen, lung,
kidney) were dissected and weighed. All tissues were treated with
HNO3 to get clear solution and then the Fe concentrations were
determined by ICP-AES.
Education Commission and Shanghai Education Development
Foundation.
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Supporting Information
Supporting Information is available from the Wiley Online Library
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Acknowledgements
This work was financially supported by the National Science Foun-
dation of China (Grant Nos. 51273047 and 51473037) and the
© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
small 2016,
DOI: 10.1002/smll.201601094