Determination of the time course of an enzymatic reaction by 1H NMR spectroscopy: Hydroxynitrile lyase catalysed transhydrocyanation

Article abstract of DOI:10.1016/0584-8539(95)01574-4

The time course of the enzyme catalysed transhydrocyanation of benzaldehyde to give (S)-mandelonitrile was investigated using a hydroxynitrile lyase from Hevea brasiliensis as catalyst and acetone cyanohydrin as cyanide donor. Employing special techniques it was possible to apply ¹H NMR spectroscopy in aqueous medium to monitor the concentration changes of all substrates and products. By this technique strong evidence for inhibition of the enzyme at higher substrate concentrations was obtained.