BOROHYDRIDE REDUCTION OF PERIODATE-OXIDIZED NUCLEOTIDES; ISOLATION AND STRUCTURE OF THE REDUCTION INTERMEDIATE

Article abstract of DOI:10.1016/0008-6215(82)85009-X

The reduction of periodate-oxidized nucleotides with sodium borohydride proceeds via a reaction intermediate presumed to be a monoalcohol.The borohydride-reduction intermediate of periodate-oxidized ADP has been isolated by anionexchange, liquid chromatography, and subjected to further reduction.Using sodium borohydride and sodium borodeuteride alternately in the two reduction steps, it was determined, by 1H-n.m.r.-spectral analysis, that the two aldehyde groups are sequentially reduced in the order 3' and 2', and it was concluded that the isolated intermediate corresponds to the semi-reduced, 3'-alcohol, 2'-aldehyde derivative.This compound should be a useful analog for the study of enzymes and proteins that interact with nucleotides.