Preparation and Enzymatic Hydrolysis of Polymers Anchoring Biphenyl-2-ol

Article abstract of DOI:10.1246/bcsj.58.1182

Polymers anchoring fungicidal biphenyl-2-ol(BPOL) were prepared by the polymerization and copolymerization of 2-biphenylyl methacrylate(BPMA) with various comonomers such as styrene, vinyl acetate, 2-hydroxyethyl methacrylate, N-methacryloylglycine, and N-methacryloyl-DL-alanine.The reactivity ratios of BPMA with the comonomers were evaluated.The α-chymotrypsin-catalyzed hydrolysis of the polymers was also studied.The release of BPOL from the polymers was represented by means of the Michaelis constant K_m and the catalytic-rate constant k_cat.The BPMA homopolymer gave higher K_m and k_cat values than the copolymers, and the copoly mers with a higher content of substrate units underwent cleavage more readily.It was found that the hydrolysis of the polymeric substrates is affected by the structure and character of the comonomer rather than the arrangement order of the repeating monomer units in the polymer sequence.