Glycolic acid production using ethylene glycol-oxidizing microorganisms

Article abstract of DOI:10.1271/bbb.65.2265

Screening for microorganisms oxidizing ethylene glycol to glycolic acid was carried out. Among stock cultures, several yeasts and acetic acid bacteria showed high glycolic acid producing activity. Pichia naganishii AKU 4267 formed the highest concentration of glycolic acid, 35.3 g/l, from 10% (v/v) ethylene glycol (molar conversion yield, 26.0%). Among soil isolates, Rhodotorula sp. 3Pr-126, isolated using propylene glycol as a sole carbon source, formed the highest concentration of glycolic acid, 25.1 g/l, from 10% (v/v) ethylene glycol (molar conversion yield, 18.5%). Rhodotorula sp. 3Pr-126 showed higher activity toward 20% (v/v) ethylene glycol than P. naganishii AKU 4267. Optimization of the conditions for glycolic acid production was investigated using P. naganishii AKU 4267 and Rhodotorula sp. 3Pr-126. Under the optimized conditions, P. naganishii AKU 4267 and Rhodotorula sp. 3Pr-126 formed 105 and 110 g/l of glycolic acid (corrected molar conversion yields, 88.0 and 92.2%) during 120 h of reaction, respectively.

Full text of DOI:10.1271/bbb.65.2265

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Glycolic Acid Production Using Ethylene Glycol-
Oxidizing Microorganisms
Michihiko KATAOKAa, Mié SASAKIa, Aklani-Rose G.D. HIDALGOa, Michiko NAKANOa &
Sakayu SHIMIZUa
a Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Published online: 22 May 2014.
To cite this article: Michihiko KATAOKA, Mié SASAKI, Aklani-Rose G.D. HIDALGO, Michiko NAKANO & Sakayu SHIMIZU (2001)
Glycolic Acid Production Using Ethylene Glycol-Oxidizing Microorganisms, Bioscience, Biotechnology, and Biochemistry,
65:10, 2265-2270, DOI: 10.1271/bbb.65.2265
To link to this article: http://dx.doi.org/10.1271/bbb.65.2265
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