Derivatization of Vinyl Aldehydes with Anthrone Prior to High-Performance Liquid Chromatography with Fluorometric Detection

Article abstract of DOI:10.1021/ac00158a004

Precolumn high-performance liquid chromatography derivatization of several vinyl aldehydes, specifically acrolein, crotonaldehyde, and methacrolein, has been studien by use of the reagent anthrone.After selective condensation of these α or β-unsaturated aldehydes to form fluorescent benzanthrone derivatives, separation of these compounds was facile on a 10-cm C-18 column.Although ultraviolet detection was possible, fluorescent detection proved more versatile and sensitive.Four liquor samples were subsequently assayed for acrolein and crotonaldehyde.Linearity for these compounds in alcoholic solutions ranged from 0.02 to at least 14 ppm with detection limits reaching down to 0.005pmm.