Remarkable Enhancement of 7-Ethoxycoumarin O-Deethylation by Lys250, Arg251 and Lys253 Mutations of Cytochrome P450 1A2

Article abstract of DOI:10.1246/cl.1994.311

Catalytic efficiency, k_cat/K_m, for 7-ethoxycoumarin O-deethylation was remarkably enhanced up to 6-fold by mutations at Lys250, Arg251 or Lys253 in cytochrome P450 1A2, while that for methanol hydroxylation was not changed by the same mutations.It is thus suggested that this ionic site is located at or near one of the hydrophobic substrate-interacting sites of this enzyme.