MgSO4

THF NMR spectra Chemical shifts NMR Amine Column chromatography Silica gel Sodium bicarbonate Melting point Coupling constants Chiral EtOAc 1H NMR Et2O MeOH HCl brine 13C NMR DOI reflux Chromatography Optimization Enantioselective synthesis CDCl3 H2SO4 Hz Ethyl acetate Aldehyde Rotary evaporation DMSO TMS H2O TFA Enone MS Ketone CB2 receptor Structure-Activity Relationship (SAR) Analysis Chemical Synthesis High-Throughput Screening In Vivo Testing Identify key structural features Preclinical and Clinical Development Regulatory Approval Iterative Design Post-Market Surveillance Dopamine D2 Receptor Bioassays and Testing Structure Elucidation Target Selection and Validation