Preclinical and Clinical Development

Synthesis Chemical shifts NMR Column chromatography Sodium bicarbonate Melting point Coupling constants brine MgSO4 Molecular docking reflux Optimization CDCl3 Ethyl acetate Rotary evaporation DMSO TMS H2O TFA nonpeptide inhibitors binding affinity X-ray crystallography Structure-Activity Relationship (SAR) Analysis Chemical Synthesis In vitro Testing Biological Assays Structure-Activity Relationship (SAR) Studies High-Throughput Screening In Vivo Testing Literature Review Lead Optimization Hit Identification Virtual Screening De Novo Design Regulatory Approval Design and Retrosynthetic Analysis Structure-Activity Relationship (SAR) SAR Analysis Functional Group Introduction Compound Synthesis Pharmacokinetic and Toxicity Studies Scaffold Design Post-Market Surveillance Linker Attachment Bioassays and Testing Structure Elucidation Target Selection and Validation Design and Rationalization Target Identification X-ray Structure Determination