1017-76-1Relevant articles and documents
A Biocatalytic Cascade for Versatile One-Pot Modification of mRNA Starting from Methionine Analogues
Muttach, Fabian,Rentmeister, Andrea
, p. 1917 - 1920 (2016)
Methyltransferases have proven useful to install functional groups site-specifically in different classes of biomolecules when analogues of their cosubstrate S-adenosyl-l-methionine (AdoMet) are available. Methyltransferases have been used to address different classes of RNA molecules selectively and site-specifically, which is indispensable for biophysical and mechanistic studies as well as labeling in the complex cellular environment. However, the AdoMet analogues are not cell-permeable, thus preventing implementation of this strategy in cells. We present a two-step enzymatic cascade for site-specific mRNA modification starting from stable methionine analogues. Our approach combines the enzymatic synthesis of AdoMet with modification of the 5′ cap by a specific RNA methyltransferase in one pot. We demonstrate that a substrate panel including alkene, alkyne, and azido functionalities can be used and further derivatized in different types of click reactions.
A SIMPLE PREPARATION OF S-ALKYL HOMOCYSTEINE DERIVATIVES: S-PHOSPHONOMETHYL HOMOCYSTEINES AS INHIBITORS OF GLUTAMINE SYNTHETASE
Logusch, Eugene W.
, p. 6055 - 6058 (2007/10/02)
A facile synthesis of S-alkyl homocysteines is described which features the coupling of sodium alkyl thiolates with methyl 4-bromo-2-phthalimidobutyrate, followed by hydrolysis.This approach is exemplified by the synthesis of S-phosphonomethyl homocysteine sulfone, a new inhibitor of the enzyme glutamine synthetase.
