130-49-4Relevant academic research and scientific papers
Effect of alkaline earth metal ions on the phosphodiester hydrolysis of RNA
Yashiro, Morio,Higuchi, Maiko,Washizu, Yusuke,Komiyama, Makoto
, p. 1843 - 1844 (2002)
Hydrolyses of di- and triribonucleotides assisted by alkaline earth metal ions were investigated at pH 7.3 and 50 °C. Mg2+ was the most effective for the phosphodiester hydrolysis, and the rate exhibited first-order dependence on the concentration of Mg2+ in a range of 0.1-1.0 mol dm-3.
Hetero-dinuclear metal complexes for RNA hydrolysis
Kamitani, Jun,Kawahara, Ryuto,Yashiro, Morio,Komiyama, Makoto
, p. 1047 - 1048 (1998)
In the presence of a ligand having two metal-binding sites, Fe(III)/Zn(II) and Fe(III)/Cd(II) combinations hydrolyze adenylyl(3′-5′)adenosine. The notable activities are ascribed to the synergetic cooperation between the two kinds of metal ions in the hetero-dinuclear complexes.
A Stark Contrast to Modern Earth: Phosphate Mineral Transformation and Nucleoside Phosphorylation in an Iron- and Cyanide-Rich Early Earth Scenario
Burcar, Bradley,Casta?eda, Alma,Lago, Jennifer,Daniel, Mischael,Pasek, Matthew A.,Hud, Nicholas V.,Orlando, Thomas M.,Menor-Salván, César
, p. 16981 - 16987 (2019)
Organophosphates were likely an important class of prebiotic molecules. However, their presence on the early Earth is strongly debated because the low availability of phosphate, which is generally assumed to have been sequestered in insoluble calcium and iron minerals, is widely viewed as a major barrier to organophosphate generation. Herein, we demonstrate that cyanide (an essential prebiotic precursor) and urea-based solvents could promote nucleoside phosphorylation by transforming insoluble phosphate minerals in a “warm little pond” scenario into more soluble and reactive species. Our results suggest that cyanide and its derivatives (metal cyanide complexes, urea, ammonium formate, and formamide) were key reagents for the participation of phosphorus in chemical evolution. These results allow us to propose a holistic scenario in which an evaporitic environment could concentrate abiotically formed organics and transform the underlying minerals, allowing significant organic phosphorylation under plausible prebiotic conditions.
RNA hydrolysis by the combination of the lanthanum(III) ion and hydrogen peroxide
Komiyama, Makoto,Kamitani, Jun,Sumaoka, Jim,Asanuma, Hiroyuki
, p. 869 - 870 (1996)
Diribonucleotides are efficiently hydrolyzed by the combination of the lanthanum(III) ion and hydrogen peroxide at pH 7.2 and 30 °C. The rate constant for the hydrolysis of adenylyl(3′-5′)adenosine by the mixture of La(ClO4)3 (10 mmol dm-3) and H2O2 (100 mmol dm-3) is 7.7 x 10-2 min-1 (half-life 9 min), corresponding to a 460 fold cooperative promotion of the catalysis.
Lanthanide Complexes with High Stability and Efficiency for the Hydrolysis of a Ribonucleotide Dimer
Yashiro, Morio,Ishikubo, Akira,Takarada, Tohru,Komiyama, Makoto
, p. 665 - 666 (1995)
Lanthanide complexes with N,N,N'N'-tetrakisethylenediamine (TPEN) efficiently hydrolyze a ribonucleotide dimer, ApA, at 35 deg C, pH = 7.2.The activity of La3+-TPEN is greater than of free La3+ ion.The Ln3+-TPEN complexes are sufficiently stable in neutral aqueous solutions (the formation constants are in the range log K/mol-1dm3 = 3.9 - 6.2).These complexes are promising for the active site of artificial ribonucleases.
Synergetic Catalysis by Two Non-lanthanide Metal Ions for Hydrolysis of Diribonucleotides
Irisawa, Makoto,Takeda, Naoya,Komiyama, Makoto
, p. 1221 - 1222 (1995)
Adenylyl(3'-5')adenosine and uridylyl(3'-5')uridine are efficiently hydrolysed at pH 7 by bimetallic cooperation of zinc(ii) with tin(iv), indium(iii), iron(iii) or aluminium(iii).
Catalytic Hydrolysis of 2',3'-Cyclic Adenosine Monophosphate by Aqua(2,2':6',2''-terpyridine)copper(II): Breakdown of the Analogy Between Activated Phosphodiesters and RNA
Bashkin, James K.,Jenkins, Lisa A.
, p. 3631 - 3632 (1993)
Aqua(2,2':6',2''-terpyridine)copper(II) catalyses the hydrolysis of 2',3'-cyclic adenosine monophosphate, but does not hydrolyse the 'activated' substrate bis(p-nitrophenyl) phosphate indicating that the latter is not a reliable model for biological phosphoric ester hydrolysis.
Preparation and Study of Dinuclear Zinc(II) Complex for the Efficient Hydrolysis of the Phosphodiester Linkage in a Diribonucleotide
Yashiro, Morio,Ishikubo, Akira,Komiyama, Makoto
, p. 1793 - 1794 (1995)
A dinuclear zinc(II) complex with N,N,N',N'-tetrakis-2-hydroxy-1,3-diaminopropane efficiently hydrolyses ApA at pH 7 and 50 deg C; the complex can thus be regarded as a good artificial ribonuclease which effectively mimics enzyme active sites.
Kinetic Analysis of Diamine-Catalyzed RNA Hydrolysis
Komiyama, Makoto,Yoshinari, Koichi
, p. 2155 - 2160 (1997)
The catalysis of various amines for the hydrolysis of RNA has been kinetically investigated, and the catalytic rate constants for each of the ionic states of these amines are determined. Ethylenediamine and 1,3-propanediamine are highly active under the physiological conditions, mainly because they preferentially take the catalytically active monocationic forms. The catalysis of these diamines is further promoted by the intramolecular acid-base cooperation of the neutral amine and the ammonium ion. In contrast, monoamines overwhelmingly exist at pH 7 as the inactive cations. Potential application of the catalysis by the diamines and the related oligoamines is discussed.
Stereospecific Cleavage of Adenosine 2',3'-Cyclic Monophosphate Catalyzed by Cyclodextrins
Komiyama, Makoto
, p. 689 - 692 (1988)
Stereospecific cleavage of adenosine 2',3'-cyclic monophosphate to the 2'-monophosphate is achieved at pH 11.08, 20 deg C by use of β- and γ-cyclodexrins as catalysts.The 2'/3' ratio for the product is 7.7 at the concentration 1.5x10-2 mol dm-3 of β-cyclodextrin.In contrast, α-cyclodextrin promotes the formation of the 3'-monophosphate, giving the product 2'/3' ratio 0.49 at the concentration 1.0x10-2 mol dm-3.
