80154-34-3 Usage
Brief description
Helicid is the active ingredient isolated from the fruit of proteaceae plant Helicia nilagirica, with its chemical structure being similar to gastrodin (hydroxymethyl benzene - O-β-D-glucopyranoside). Pharmacological and clinical studies have shown that helicid has similar effects on the central nervous system with gastrodin, but its sedative, euthanasia and analgesic effect is stronger than gastrodin. The efficacy on the treatment of neurosis efficacy is definite without rebound insomnia and drug dependence. Therefore, it has excellent clinical value. Its fruit tablets have been used in clinical.
Extraction and Separation
Figure 1 the process of purification of helicid from the fruit of proteaceae plant Helicia nilagirica
Determination of content
HPLC method
(1) Chromatographic conditions
Column: YWCT-C18 (25cm × 2.6mm, 10μm) high pressure grouting method. Flowing phase: methanol-water (20:80); flow rate: 0.7 ml / min; column temperature: room temperature; UV detection wavelength: 270 nm; paper speed: 2.5 mm / min.
(2) Reference solution preparation
Precisely weigh control reference substance of helicid; prepare 1 mg/mL solution using 20% methanol. Precise weigh Bergenin; dissolve in 20% methanol; apply ultrasonic solubilization, dub bergenin into about 2mg / ml internal standard solution.
(3) Preparation of sample solution
Accurately weighed dried helicid powder of about 1g, and put it into 25ml volumetric flask; add 20 mL methanol for soak of 0.5 h; ultrasonic extraction 80min, cool, set volume into 25 mL with methanol; filter and filtrate will become the sample solution.
(4) Determination
Take helicid reference solution in 5ml volumetric flask, add bergenin internal standard solution of 1.5ml; diluted with 20% methanol to the mark, mix; take 5 mL sample solution, volatilize; add 1. 5mL the internal standard solution; dilute with 20% methanol to the mark, mix well. Take 5μl of both reference substance and sample solution, inject, determine according to the chromatographic conditions; calculate the content with peak area ratio.
(5) Measurement results
Use the above internal standard method to measure the content of helicid in Samples I and II, and the results were 0.963% and 0.928%.
Its chemical structure is similar to Gastrodia; have a strong analgesic, sedation and euthanasia effect. It is used for the treatment of neurasthenia, neurasthenic syndrome, functional disorders, vascular headache and trigeminal neuralgia. It has remarkable effect on neurasthenic headache, dizziness and sleep disorders.
Chemical Properties
It appears as white needle-like crystals, mp.190 °C ~ 193 °C, [α] D20-100 ° ~ -108 ° (water).
Uses
It is used in the treatment of ischemic cardiovascular and cerebrovascular diseases. Also used in Chinese medicine in the treatment of headache and insomnia.
Check Digit Verification of cas no
The CAS Registry Mumber 80154-34-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,0,1,5 and 4 respectively; the second part has 2 digits, 3 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 80154-34:
(7*8)+(6*0)+(5*1)+(4*5)+(3*4)+(2*3)+(1*4)=103
103 % 10 = 3
So 80154-34-3 is a valid CAS Registry Number.
InChI:InChI=1/C13H16O7/c14-5-7-1-3-8(4-2-7)19-13-12(18)11(17)10(16)9(6-15)20-13/h1-5,9-13,15-18H,6H2/t9-,10-,11+,12-,13-/m1/s1
80154-34-3Relevant articles and documents
Preparation method of glucoside and derivatives thereof
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Paragraph 0044-0045; 0048, (2020/04/02)
The invention discloses a preparation method of glucoside and derivatives thereof. According to the method, all hydroxyl groups on a sugar molecule structure are acetylated, a ligand containing phenolic hydroxyl groups is prepared at the same time, then boron trifluoride-diethyl ether is used as a catalyst, the two substances are condensed to obtain tetraacetylated glucoside, and finally acetyl protecting groups are removed to obtain the required glucoside. The method can selectively catalyze hemiacetal hydroxyl of monosaccharide to react with hydroxyl to obtain glucoside, and the product is single. The method is simple in production operation and low in equipment requirement, can be used for synthesizing glucoside and derivatives thereof with similar structures, is green and environment-friendly, and can be used for large-scale production.
