81-07-2 Usage
Chemical Description
Saccharin is an artificial sweetener that is commonly used in food and drinks.
Description
Saccharin, also known as ortho-sulfobenzoic acid imide, is an organic compound that is commonly used as a non-nutritive sweetening agent. It occurs in various forms of salts, primarily calcium and sodium, and is 300-400 times sweeter than sucrose.
Used in Food Industry:
Saccharin is used as a non-nutritive synthetic sweetener for its high sweetness level, which is 300-400 times sweeter than sucrose. It is used in low-calorie foods such as jam, beverages, and desserts due to its non-nutritive nature and low sodium content, which helps reduce after-taste.
Used in Pharmaceutical Industry:
Saccharin is used as a pharmaceutic aid (flavor) in the development of medications. It was formerly listed as reasonably anticipated to be a human carcinogen but was delisted due to insufficient cancer data to meet the current criteria for this listing.
Used in Scientific Research:
Saccharin is used in high-performance liquid chromatographic methods for the simultaneous separation and determination of acesulfame potassium, saccharin, and aspartame. It is also used in sweet preference tests of rats for research purposes.
History
Saccharin was discovered in 1879 by chemists Constantin Fahlberg and Ira Remsen as they were researching about the oxidation of o-toluenesulfonamide. While eating, Fahlberg noticed the presence of sweetness in his food due to his arms and hands that contained saccharin. As he checked his laboratory apparatus by taste tests, Fahlberg found out that the source of this sweetness was from saccharin. Saccharin is still made of toluenesulfonamide and from phthalic anhydride.
History
Saccharin is the oldest and one of the best-known artificial sweeteners. It was accidentally discovered in 1878 by Ira Remsen (1846 1927) and his postdoctoral research fellow Constantin Fahlberg (1850 1910) at Johns Hopkins University when he was working on toluene derivatives from coal tar. He traced the taste back to the oxidized sulfonated chemicals he was working with and determined it was a sulfonated amide benzoic acid compound. Remsen and Fahlberg jointly published their findings on the compound in 1879 and 1880 in American and German journals. During the next several years, Remsen continued his academic work as one of the world's leading chemists, and Fahlberg perfected methods for commercialization of saccharin. Fours year after they published their work, Fahlberg and his uncle, Adolf List, applied for a United States patent for the compound, which was granted in 1885 (U.S. patent number 319082). Saccharin was first introduced to the public in 1885. It was initially promoted as an antiseptic and food preservative. The use of saccharin as a sweetener started around 1900 when it was marketed for use by people with diabetes. Because saccharin
was a cheap sugar substitute, it was viewed as a threat to the sugar industry. Sugar manufacturers
in Europe, Canada, and the United States lobbied for laws restricting saccharin’s
use.
Calls to regulate saccharin in foods have been present throughout its history. Early in the 20th
century, the political climate promoted legislation and government oversight to ensure that food
was safe. In 1906, the passage of the Federal Food and Drug Act gave government regulatory
authority concerning the safety of food. The Department of Agriculture’s Bureau of Chemistry,
the predecessor of the Food and Drug Administration (FDA), performed research and made
recommendations with respect to food additives. In 1907, a study by the newly created Board
of Food and Drug Inspection made claims (latter refuted) that saccharin damaged the kidneys
and other organs. The leader of the Bureau of Chemistry, Harvey W. Wiley (1844–1930), was a
member of the Board and held the view that saccharin (and other chemicals such as benzoates)
was dangerous.
Approximately 30,000 tons per year of saccharin and saccharin salts are
used globally each year, with about 5,000 tons of this used in the United States.
Questions on saccharin’s safety has followed its usage to the present day. Saccharin is banned
in Canada (except in special cases), several European countries, and many other countries.
Countries where it is legal place restrictions on its use. Saccharin has been regulated in the
United States since the beginning of the century. A Canadian study in 1977 that reported saccharin
Legislation, signed into law on December 21, 2000, repealed the warning label requirement for products containing saccharin.
The National Cancer
Institute’s position is that there is no clear evidence linking saccharin to cancer in humans.
Properties
Saccharin is stable when heated and does not chemically react with other food ingredients, therefore, it stores well. When blended with other sweeteners, saccharin often compensates for each sweetener’s faults and weakness. Commonly, saccharin is used with aspartate in diet carbonated soft drinks.
Saccharin is insoluble in water in its acid form. Its majorly used form as an artificial sweetener is its sodium salt.
Safety and Health Effects
The utilization of saccharin in human food has raised numerous health and safety concerns. In the 1970s, saccharin was linked with the development of bladder in rodents in various laboratory studies on rats. Consequently, the United States Food and Drug Administration (FDA) pushed for its ban, sighting that it is carcinogenic to humans.
However, after strong objection from the public regarding the ban, American Congress intervened and allowed the compound to remain in the food supply as long as all the manufactures libel it with a warning when packaging.
Saccharin gas been classified to have no nutritional or food energy value, as such, it safe for patients with diabetes.
Production Methods
Saccharin is prepared from toluene by a series of reactions known as the Remsen–Fahlberg method. Toluene is first reacted with chlorosulfonic acid to form o-toluenesulfonyl chloride, which is reacted with ammonia to form the sulfonamide. The methyl group is then oxidized with dichromate, yielding o-sulfamoylbenzoic acid, which forms the cyclic imide saccharin when heated.
An alternative method involves a refined version of the Maumee process. Methyl anthranilate is initially diazotized to form 2- carbomethoxybenzenediazonium chloride; sulfonation followed by oxidation then yields 2-carbomethoxybenzenesulfonyl chloride. Amidation of this material, followed by acidification, forms insoluble acid saccharin.
Preparation
Saccharin is synthesized using two methods: the Remsen-Fahlberg process and the Maumee or Sherwin-Williams method. The Remsen-Fahlberg synthesis of saccharin starts by reacting toluene with chlorosulfonic acid to give ortho and para forms of toluene-sulfonic acid (Figure 78.1). The acid can be converted to sulfonyl chlorides by treating with phosphorus pentachloride. The ortho form, o-toluene-sulfonyl chloride, is treated with ammonia to give o-toluene-sulfonamide, which is then oxidized with potassium permanganate to produce o-sulfamido-benzoic acid. On heating, the latter yields saccharin. Another synthesis was developed at Maumee Chemical Company in Toledo, Ohio, and it came to be known as the Maumee process. This process starts with phthalic anhydride, which is converted into anthranilic acid. Anthranilic acid is then reacted with nitrous acid, sulfur dioxide, chlorine, and ammonia to give saccharin. The Maumee process was further refi ned by the Sherwin-Williams Company and is therefore now referred to as the Sherwin-Williams process.
Air & Water Reactions
Slightly soluble in water.
Reactivity Profile
An amide. Acid to litmus. pH of 0.35% aqueous solution: 2.0. Organic amides/imides react with azo and diazo compounds to generate toxic gases. Flammable gases are formed by the reaction of organic amides/imides with strong reducing agents. Amides are very weak bases (weaker than water). Imides are less basic yet and in fact react with strong bases to form salts. That is, they can react as acids. Mixing amides with dehydrating agents such as P2O5 or SOCl2 generates the corresponding nitrile. The combustion of these compounds generates mixed oxides of nitrogen (NOx).
Hazard
A questionable carcinogen. Products con-
taining it must have a warning label.
Fire Hazard
Flash point data for Saccharin are not available; however, Saccharin is probably combustible.
Flammability and Explosibility
Nonflammable
Pharmaceutical Applications
Saccharin is an intense sweetening agent used in beverages, food
products, table-top sweeteners, and oral hygiene products such as
toothpastes and mouthwashes. In oral pharmaceutical formulations,
it is used at a concentration of 0.02–0.5% w/w. It has been
used in chewable tablet formulations as a sweetening agent.
Saccharin has been used to form various pharmaceutical cocrystals.
Saccharin can be used to mask some unpleasant taste characteristics
or to enhance flavor systems. Its sweetening power is
approximately 300–600 times that of sucrose.
Biochem/physiol Actions
A sweet tastant for mammals. A glycerol taste receptor binding site specific for glucose has been proposed in drosophila.
Safety Profile
Confirmed carcinogen withexperimental neoplastigenic and tumorigenic data. Mildacute toxicity by ingestion. Experimental teratogenic andreproductive effects. Mutation data reported. Whenheated to decomposition it emits toxic NOx and SOx.
Safety
There has been considerable controversy concerning the safety of
saccharin, which has led to extensive studies since the mid-1970s.
Two-generation studies in rats exposed to diets containing
5.0–7.5% total saccharin (equivalent to 175 g daily in humans)
suggested that the incidence of bladder tumors was significantly
greater in saccharin-treated males of the second generation than in
controls. Further experiments in rats suggested that a contaminant
of commercial saccharin, o-toluene sulfonamide, might
also account for carcinogenic effects. In view of these studies, a ban
on the use of saccharin was proposed in several countries. However,
in 1977 a ban by the FDA led to a Congressional moratorium that
permitted the continued use of saccharin in the USA.
From the available data it now appears that the development of
tumors is a sex-, species-, and organ-specific phenomenon, and
extensive epidemiological studies have shown that saccharin intake
is not related to bladder cancer in humans.
The WHO has set a temporary acceptable daily intake for
saccharin, including its calcium, potassium, and sodium salts, at up
to 2.5 mg/kg body-weight. In the UK, the Committee on Toxicity
of Chemicals in Food, Consumer Products, and the Environment
(COT) has set an acceptable daily intake for saccharin and its
calcium, potassium, and sodium salts (expressed as saccharin
sodium) at up to 5 mg/kg body-weight.
Adverse reactions to saccharin, although relatively few in
relation to its widespread use, include: urticaria with pruritus
following ingestion of saccharin-sweetened beverages and
photosensitization reactions.
LD50 (mouse, oral): 17.5 g/kg
LD50 (rat, IP): 7.10 g/kg
LD50 (rat, oral): 14.2 g/kg
storage
Saccharin is stable under the normal range of conditions employed
in formulations. In the bulk form it shows no detectable
decomposition and only when it is exposed to a high temperature
(125°C) at a low pH (pH 2) for over 1 hour does significant
decomposition occur. The decomposition product formed is
(ammonium-o-sulfo)benzoic acid, which is not sweet. The
aqueous stability of saccharin is excellent.
Saccharin should be stored in a well-closed container in a dry
place.
Shipping
UN3077 Environmentally hazardous substances,
solid, n.o.s., Hazard class: 9; Labels: 9-Miscellaneous haz-
ardous material, Technical Name Required.
Purification Methods
Purify saccharin by recrystallisation from Me2CO [solubility 7.14% at 0o, 14.4% at 50o], or aqueous isoPrOH to give a fluorescent solution. It sublimes in vacuo. It is an artificial sweetner and is 500 times sweeter than sucrose. [DeGarmo et al. J Am Pharm Assoc (Sci Ed) 41 17 1952, Beilstein 27 H 168, 870, 27 I 266, 27 II 214, 27 III/IV 2649.]
Incompatibilities
Saccharin can react with large molecules, resulting in a precipitate
being formed. It does not undergo Maillard browning.
Regulatory Status
Accepted for use as a food additive in Europe. Note that the EU
number ‘E954’ is applied to both saccharin and saccharin salts.
Included in the FDA Inactive Ingredients Database (oral solutions,
syrups, tablets, and topical preparations). Included in nonparenteral
medicines licensed in the UK. Included in the Canadian List of
Acceptable Non-medicinal Ingredients.
Check Digit Verification of cas no
The CAS Registry Mumber 81-07-2 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 8 and 1 respectively; the second part has 2 digits, 0 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 81-07:
(4*8)+(3*1)+(2*0)+(1*7)=42
42 % 10 = 2
So 81-07-2 is a valid CAS Registry Number.
InChI:InChI=1/C7H5NO3S/c9-7-5-3-1-2-4-6(5)12(10,11)8-7/h1-4H,(H,8,9)
81-07-2Relevant articles and documents
Synthesis, characterization and antimicrobial evaluation of new 3-(Alkyl/Arylamino)benzo[d]isothiazole 1,1-derivatives
Kamble, Dhanraj P.,Shankarwar, Anil G.,Mane, Yogesh D.,Tigote, Radhakrishna M.,Sarnikar, Yuvaraj P.,Madje, Balaji R.
, p. 797 - 804 (2021/09/08)
The saccharine nucleus has long been recognized as a significant component in medicine. A series of pseudo-saccharine amines derivatives (7a-j) were synthesized and examined for their antibacterial activity. After testing all compounds, 7b, 7f, 7g, 7i and 7j were found most effective against Escherichia coli, Streptococcus aureus and Bacillus subtilis strains. The MIC of the compound was found from 4.6 to 16.1 μM. Further, compound 7f and 7i exhibited excellent activity against E.coli and Bacillus subtilis with MIC value 4.6 and 4.7 μM respectively. The compound 7b and 7i was found active against all the three bacteria. The zone inhibition was observed at 10 μM against Escherichia coli, Staphylococcus aureus and Bacillus subtilis at 0.9, 1.8, 3.9 respectively for 7b and 1.0, 1.8 and 2.0 cm respectively for 7i.
Preparation method of saccharin
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Paragraph 0128-0137; 0140-0151, (2020/10/14)
The invention discloses a preparation method of saccharin. The invention provides a preparation method of saccharin as shown in a formula 1 represented in the specification. The preparation method ischaracterized by comprising the following step: in water, in the presence of tungstate and/or tungstic acid, carrying out oxidation reaction on a compound shown in a formula 2 and hydrogen peroxide toobtain saccharin shown in a formula 1.
CARBON MONOXIDE PRODRUGS FOR THE TREATMENT OF MEDICAL DISORDERS
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Page/Page column 128-131, (2020/05/21)
The present invention provides new compounds and compositions thereof that release carbon monoxide for the treatment of medical disorders that are responsive to carbon monoxide, for example, inflammatory, pain, and dermatological disorders.
Xn:Harmful;
