Gas chromatography of volatile amino acid derivatives

Add time:07/27/2019    Source:sciencedirect.com

Methods are described for the preparation of volatile derivatives of amino acids, which permits analysis by gas chromatography. Alanine, glycine, isoleucine, leucine, serine, threonine and valine were converted to the trifluoroacetyl amino acid n-amyl esters, and the separation of these seven compounds was investigated on a wide variety of stationary phases. No single phase capable of achieving complete and unequivocal resolution was found, but the separation necessary for quantitative work was obtained by the use of two columns packed with different phases.

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