A rapid method for measuring intracellular pH using BCECF-AM (cas 134331-92-3)

Add time:07/30/2019    Source:sciencedirect.com

A rapid intracellular pH (pHi) measurement method based on initial rate of increase of fluorescence ratio of 2′,7′-bis(2-carboxyethyl)-5,6-carboxyfluorescein upon dye addition to a cell suspension in growth medium is reported. A dye transport model that describes dye concentration and fluorescence values in intracellular and extracellular spaces provides the mathematical basis for the approach. Experimental results of ammonium chloride challenge response of the two suspension cells, Spodoptera frugiperda and Chinese hamster ovary (CHO) cells, successfully compared with results obtained using traditional perfusion method. Since the cell suspension does not require any preparation, measurement of pHi can be completed in about 1 min minimizing any potential errors due to dye leakage.

We also recommend Trading Suppliers and Manufacturers of BCECF-AM (cas 134331-92-3). Pls Click Website Link as below: cas 134331-92-3 suppliers

Prev:Use of BCECF and propidium iodide to assess membrane integrity of acutely isolated CA1 neurons from rat hippocampus
Next:The fluorescent probe BCECF has a heterogenous distribution in sea urchin eggs)