The fluorescent probe BCECF has a heterogenous distribution in sea urchin eggs

Add time:07/31/2019    Source:sciencedirect.com

Sea urchin eggs were loaded with the pH-sensitive fluorescent probe BCECF. Homogenization of these eggs, followed by centrifugation, resulted in 25% of the total homogenate fluorescence remaining with the pellet. Fluorescence microscopy revealed brightly fluorescing punctate organelles whose fluorescence was not quenched by acidification. The excitation spectrum of intracellular BCECF was markedly red-shifted compared to probe calibrated in buffer. Model excitation spectra based on a two compartment model mimicked the intracellular BCECF spectrum, therefore, supporting the possibility that organelles in sea urchin eggs accumulate large amounts of BCECF in a relatively pH-insensitive form.

We also recommend Trading Suppliers and Manufacturers of BCECF-AM (cas 134331-92-3). Pls Click Website Link as below: cas 134331-92-3 suppliers

Prev:A rapid method for measuring intracellular pH using BCECF-AM (cas 134331-92-3)
Next:Analysis of the uptake of the fluorescent marker 2′,7′-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis)