Mass Spectrometric Analysis of Androstan-17β-ol-3-one and Androstadiene-17β-ol-3-one Isomers

Add time:07/26/2019    Source:sciencedirect.com

Mass spectrometric identification and characterization of steroids using electrospray ionization and tandem mass spectrometry has advantages in drug testing and doping control analysis attributable to limitations of gas chromatography followed by electron ionization mass spectrometry. Steroids with an androstadiene-17β-ol-3-one nucleus and double bonds located either at C-1 and C-4, C-4 and C-9, or C-4 and C-6 were used to determine characteristic fragmentation pathways. Diagnostic dissociation routes are proposed using deuterium labeling, MS3 experiments, and analyses of structurally closely related compounds. Steroids such as boldenone (androst-1,4-diene-17β-ol-3-one) produced characteristic product ions at m/z 121, 135, and 147. Compounds with double bonds at C-4 and C-9 generated abundant product ions at m/z 145 and 147. Conjugated double bonds at C-4 and C-6 gave rise to an intense and characteristic signal at m/z 133. Stereochemical differentiation between 5α- and 5β-isomers of androstan-17β-ol-3-ones was possible because of significant differences in relative abundance of product ions generated by elimination of acetone from α,β-saturated 3-keto steroids.

We also recommend Trading Suppliers and Manufacturers of Androstan-3-one (cas 1224-95-9). Pls Click Website Link as below: cas 1224-95-9 suppliers

Prev:Solvent effects on infrared spectroscopy of 17β-hydroxy-17-methyl-2-oxa-5α-Androstan-3-one (cas 1224-95-9) in single solvents
Next:Metabolism of 5α-androstane-3β,17β-diol to 17β-hydroxy-5α-Androstan-3-one (cas 1224-95-9) and 5α-androstan-3α,17β-diol in the rat)